Difference between revisions of "Synthetic Biology Standardization Debate"

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==== Microscopy Standardization ====
 
==== Microscopy Standardization ====
(Stefano Insert your ideas here)
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# what reporters are more suitable for bulk measure? or single-cell? or flow cytometry?
 
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# how reporter half-life, maturation time, brightness, predisposition to form aggregates affect measurements?
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# what about multicolor imaging? how we solve the close emission spectra of the different red-emitting reporters?
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# what about degradation tags, can they turn out to be useful for programmed and precise control of reporters half-life?
  
 
=== Standardization of Gene Expression ===
 
=== Standardization of Gene Expression ===

Latest revision as of 09:38, 14 July 2009

This page collects thoughts and materials for a debate between the Arkin and Endy labs about standardization in Synthetic Biology.

Ideas to Debate

Standardization of Measurement

Microscopy Standardization

  1. what reporters are more suitable for bulk measure? or single-cell? or flow cytometry?
  2. how reporter half-life, maturation time, brightness, predisposition to form aggregates affect measurements?
  3. what about multicolor imaging? how we solve the close emission spectra of the different red-emitting reporters?
  4. what about degradation tags, can they turn out to be useful for programmed and precise control of reporters half-life?

Standardization of Gene Expression

Transcriptional Standardization

  • Can this be standardized?

Translational Standardization

  • Can this be standardized?

Transcript Degradation Standardization

  • Can this be standardized?

Protein Degradation Standardization

  • Can this be standardized?

Standardization of 'Format'

Plasmid Standardization

  • Which origins?
  • Do we have data on plasmid localization?

Genome Insertion Standardization

  • Do we have a standard locus for insertion?

Strain Standardization

  • Should we be working in the same genetic background?