Synthetic Biology:BioBricks
This is a portal for information relating to the Registry of Standard Biological Parts. Please add any information relevant to BioBricks protocols and standards.
Introduction
The Registry of Standard Biological Parts is a collection of parts: sequences of DNA with specific function that can be combined together to implement more complex functions. These parts are called BioBricks.
Standards of practice
Along with this collection of parts, it is useful to have a set of standards of practice that govern the engineering of biological systems. There have been some initial attempts to brainstorm what form those standards might take. It is likely that we will need several classes of standards and that each class of standards which have different versions to meet different needs.
- Design: the way(s) in which systems are designed from BioBrick parts.
- See Abstraction hierarchy for a discussion on how to cope with the design of increasingly complex biological systems.
- Fabrication: the way(s) in which BioBricks are physically constructed and assembled together.
- See Part fabrication for information on construction of new biological parts.
- See BioBricks construction tutorial, 3A assembly or Silver lab assembly strategy for information on assembly of existing biological parts.
- See Vectors for information on the vectors in which BioBricks reside.
- Documentation: the way(s) in which BioBricks are documented.
- See Barcodes for information on signing BioBricks parts.
- See Device datasheet example for a working example of documenting a BioBrick.
- See Semantic web ontology for an ontology for biological parts.
- Characterization: the way(s) in which the behavior of BioBricks are measured.
- See Parts characterization for some preliminary notes on parts characterization.
- See Standardized GFP quantification for notes on making absolute molecule number measurements.
- Operation: the conditions under which systems from BioBricks are operated.
- See Vectors for information on the vectors in which BioBricks reside.
- See Standard E. coli Strain for BioBricks for notes on constructing a novel strain in which all BioBricks would operate.
Protocols
Information
Projects
- Barcodes
- Device datasheet example
- Single copy vectors
- Standard E. coli Strain for BioBricks
- Standardized GFP quantification
Discussions
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