Difference between revisions of "Swartz:Protocols"

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=Protocols=
 
=Protocols=
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==Lab Ordering==
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*[https://docs.google.com/spreadsheet/viewform?formkey=dGVtSVdMVnpiWWtGbU9FVjF6TUhPb2c6MQ#gid=0 USE THIS FORM]
  
 
==Cloning==
 
==Cloning==
 
:*[https://docs.google.com/document/d/1KHeoV1WNDpWqD-90k0X5MtVM1naTK5_81uctSkKF5z0/edit Swartz Lab Molecular Biology Handbook]
 
:*[https://docs.google.com/document/d/1KHeoV1WNDpWqD-90k0X5MtVM1naTK5_81uctSkKF5z0/edit Swartz Lab Molecular Biology Handbook]
 
:*[https://docs.google.com/spreadsheet/ccc?key=0AuGtz3V56Y9xdGQyaEUtM1p1TTh1dmQtZHhLUWVpclE&pli=1#gid=5 Restriction Enzyme Library]
 
:*[https://docs.google.com/spreadsheet/ccc?key=0AuGtz3V56Y9xdGQyaEUtM1p1TTh1dmQtZHhLUWVpclE&pli=1#gid=5 Restriction Enzyme Library]
 +
:*[[Gibson_Assembly]]
 
'''DNA Design'''
 
'''DNA Design'''
 
:*[[Swartz:Protocols/Primer Design|Primer Design]]
 
:*[[Swartz:Protocols/Primer Design|Primer Design]]
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:*[[Swartz:Protocols/Quikchange|Quikchange]]
 
:*[[Swartz:Protocols/Quikchange|Quikchange]]
 
:*[https://docs.google.com/document/d/17lf6hG6u25vGYEW3y1CxoGyKHr-yJv9LbZeoYSGOw04/edit Gel Purification/PCR Cleanup]
 
:*[https://docs.google.com/document/d/17lf6hG6u25vGYEW3y1CxoGyKHr-yJv9LbZeoYSGOw04/edit Gel Purification/PCR Cleanup]
:*[[/Column_Regeneration|Column Regeneration]]
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:*[[/Column_Regeneration|MaxiPrep Column Regeneration]]
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:*[[Genomic DNA Extraction|Genomic DNA Extraction]]
 
'''Expression Templates'''
 
'''Expression Templates'''
 
:*
 
:*
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'''Organisms'''
 
'''Organisms'''
 
:*[[Transforming chemically competent cells|E.coli Transformation]]
 
:*[[Transforming chemically competent cells|E.coli Transformation]]
 
 
:*[[/Cloning| Other Cloning Protocols]]
 
:*[[/Cloning| Other Cloning Protocols]]
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:*[[Making_a_long_term_stock_of_bacteria| Creating an E. coli glycerol stock]]
  
 
==Cell Free Protein Synthesis==
 
==Cell Free Protein Synthesis==
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:*[[/Western|Western Blotting]]
 
:*[[/Western|Western Blotting]]
 
:*[[/HD_Extract_Prep|High Density Fermentation]]
 
:*[[/HD_Extract_Prep|High Density Fermentation]]
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:*[[/otDNA|Orthogonal tDNA template prep]]
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:*[[/Protein_gels|Running a protein gel]]
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**[[S30_buffer]]
  
 
==Protein Purification==
 
==Protein Purification==
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:*[[/Ribosome_binding| Spin assay for ribsome binding]]
 
:*[[/Ribosome_binding| Spin assay for ribsome binding]]
 
:*[[/HPLC|HPLC]]
 
:*[[/HPLC|HPLC]]
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:*[[Free_Sulfhydryl_Determination| Ellman's Reagent Test]]
 
:*[[Swartz:Protocols/Cytochrome C|Cytochrome C ]]
 
:*[[Swartz:Protocols/Cytochrome C|Cytochrome C ]]
 
:*[[Swartz:Protocols/Methyl Viologen|Methyl Viologen]]
 
:*[[Swartz:Protocols/Methyl Viologen|Methyl Viologen]]

Latest revision as of 13:57, 12 October 2012

Research Papers People Contact


Getting settled in the Swartz Lab


Protocols

Lab Ordering

Cloning

DNA Design

Gene Synthesis

Expression Templates

Plasmids

Organisms

Cell Free Protein Synthesis

Protein Purification

Assays

Miscellaneous

Solutions