Difference between revisions of "Swartz:Protocols"

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==Protocols==
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=Protocols=
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==Lab Ordering==
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*[https://docs.google.com/spreadsheet/viewform?formkey=dGVtSVdMVnpiWWtGbU9FVjF6TUhPb2c6MQ#gid=0 USE THIS FORM]
  
===Cloning===
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==Cloning==
:*[https://docs.google.com/document/d/1KHeoV1WNDpWqD-90k0X5MtVM1naTK5_81uctSkKF5z0 Swartz Lab Molecular Biology Handbook]
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:*[https://docs.google.com/document/d/1KHeoV1WNDpWqD-90k0X5MtVM1naTK5_81uctSkKF5z0/edit Swartz Lab Molecular Biology Handbook]
 
:*[https://docs.google.com/spreadsheet/ccc?key=0AuGtz3V56Y9xdGQyaEUtM1p1TTh1dmQtZHhLUWVpclE&pli=1#gid=5 Restriction Enzyme Library]
 
:*[https://docs.google.com/spreadsheet/ccc?key=0AuGtz3V56Y9xdGQyaEUtM1p1TTh1dmQtZHhLUWVpclE&pli=1#gid=5 Restriction Enzyme Library]
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:*[[Gibson_Assembly]]
 
'''DNA Design'''
 
'''DNA Design'''
 
:*[[Swartz:Protocols/Primer Design|Primer Design]]
 
:*[[Swartz:Protocols/Primer Design|Primer Design]]
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:*[[Swartz:Protocols/Restriction Cloning|Restriction Enzyme Digestion/Ligations]]
 
:*[[Swartz:Protocols/Restriction Cloning|Restriction Enzyme Digestion/Ligations]]
 
:*[[Swartz:Protocols/Quikchange|Quikchange]]
 
:*[[Swartz:Protocols/Quikchange|Quikchange]]
:*[[Swartz:Protocols/Gel_purification|Gel Purification]]
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:*[https://docs.google.com/document/d/17lf6hG6u25vGYEW3y1CxoGyKHr-yJv9LbZeoYSGOw04/edit Gel Purification/PCR Cleanup]
:*[[/Column_Regeneration|Column Regeneration]]
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:*[[/Column_Regeneration|MaxiPrep Column Regeneration]]
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:*[[Genomic DNA Extraction|Genomic DNA Extraction]]
 
'''Expression Templates'''
 
'''Expression Templates'''
 
:*
 
:*
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'''Organisms'''
 
'''Organisms'''
 
:*[[Transforming chemically competent cells|E.coli Transformation]]
 
:*[[Transforming chemically competent cells|E.coli Transformation]]
 
 
:*[[/Cloning| Other Cloning Protocols]]
 
:*[[/Cloning| Other Cloning Protocols]]
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:*[[Making_a_long_term_stock_of_bacteria| Creating an E. coli glycerol stock]]
  
===Cell Free Protein Synthesis===
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==Cell Free Protein Synthesis==
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:*[[/Linear_DNA_Template|Preparing linear DNA template for CFPS]]
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:*[[/1L_extract_prep|1L Extract Prep]]
 
:*[[/Cell_Free|Cell Free Protein Synthesis]]
 
:*[[/Cell_Free|Cell Free Protein Synthesis]]
 
:*[[/Autoradiogram|How to do an Autoradiogram]]
 
:*[[/Autoradiogram|How to do an Autoradiogram]]
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:*[[/Western|Western Blotting]]
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:*[[/HD_Extract_Prep|High Density Fermentation]]
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:*[[/otDNA|Orthogonal tDNA template prep]]
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:*[[/Protein_gels|Running a protein gel]]
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**[[S30_buffer]]
  
===Assays===
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==Protein Purification==
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:*[[/Purification_of_His-tagged_proteins|His-Tag Purification]]
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:*[[/Ribosome_purification|Ribosome purification]]
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==Assays==
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:*[[Nanodrop]]
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:*[[DNA_Quantification | Using the spec for DNA Quantification]]
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:*[[/Mass_spec| Sample prep for MS]]
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:*[[/Ribosome_binding| Spin assay for ribsome binding]]
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:*[[/HPLC|HPLC]]
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:*[[Free_Sulfhydryl_Determination| Ellman's Reagent Test]]
 
:*[[Swartz:Protocols/Cytochrome C|Cytochrome C ]]
 
:*[[Swartz:Protocols/Cytochrome C|Cytochrome C ]]
 
:*[[Swartz:Protocols/Methyl Viologen|Methyl Viologen]]
 
:*[[Swartz:Protocols/Methyl Viologen|Methyl Viologen]]
 
:*[[Swartz:Protocols/H2 Production (GC)|H2 Production (GC)]]
 
:*[[Swartz:Protocols/H2 Production (GC)|H2 Production (GC)]]
  
===Miscellaneous===
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==Miscellaneous==
:*[https://docs.google.com/document/d/1nlZCY_zG3eyBWw3DReVQHMm5oV88eX7cJpdavRdB2cw/edit Anaerobic Protein Production]
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:*[https://docs.google.com/document/d/1nlZCY_zG3eyBWw3DReVQHMm5oV88eX7cJpdavRdB2cw/edit Anaerobic Protein Production in E.coli]
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:*[[/A20_cells|Cell culture for A20 B-cells]]
  
 
==Solutions==
 
==Solutions==
 
:*[[/Buffers|Buffer Recipes]]
 
:*[[/Buffers|Buffer Recipes]]
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[[Category:Swartz_Protocols]]

Latest revision as of 13:57, 12 October 2012