Suggs:Protocols: Difference between revisions
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===MSC media preparation=== | ===MSC media preparation=== | ||
{| border="1" cellpadding="5" | |||
!<u>Item</u> || <u>[Stock]</u> !! <u>[Media]</u> !! <u>Amt/500mL</u> !! <u>Store</u> | |||
|- | |||
!Dexamethasone | |||
|.25 nM | |||
|.05 nM | |||
|100 uL | |||
|(-80) | |||
|- | |||
!DMEM | |||
|1X | |||
|.6X | |||
|300 mL | |||
|(4) | |||
|- | |||
!EGF | |||
|10 ug/mL | |||
|10 ng/mL | |||
|500 uL | |||
|(-80) | |||
|- | |||
!FCS | |||
|100% | |||
|1% | |||
|10 mL | |||
|(-20) | |||
|- | |||
!ITS | |||
|100% | |||
|1% | |||
|5 mL | |||
|(4) | |||
|- | |||
!LA-BSA | |||
|100 mg/mL | |||
|1 mg/mL | |||
|5 mL | |||
|(4) | |||
|- | |||
!L-ascorbic acid salt | |||
|10 nM | |||
|.1 nM | |||
|5 mL | |||
|(-20) | |||
|- | |||
!L-glutamine | |||
|200 nM | |||
|2 nM | |||
|5 mL | |||
|(-20) | |||
|- | |||
!MCDB 201 Medium | |||
|1X | |||
|.4X | |||
|200 mL | |||
|(4) | |||
|- | |||
!Pen/strep | |||
|100X | |||
|1X | |||
|5 mL | |||
|(-20) | |||
|- | |||
!PDGF | |||
|10 ug/mL | |||
|10 ng/mL | |||
|500 uL | |||
|(-80) | |||
|- | |||
|} | |||
Filter at end. | |||
===MSC subculturing=== | ===MSC subculturing=== | ||
===MSC freezing=== | ===MSC freezing=== | ||
Revision as of 13:43, 9 September 2005
MSC media preparation
| Item | [Stock] | [Media] | Amt/500mL | Store |
|---|---|---|---|---|
| Dexamethasone | .25 nM | .05 nM | 100 uL | (-80) |
| DMEM | 1X | .6X | 300 mL | (4) |
| EGF | 10 ug/mL | 10 ng/mL | 500 uL | (-80) |
| FCS | 100% | 1% | 10 mL | (-20) |
| ITS | 100% | 1% | 5 mL | (4) |
| LA-BSA | 100 mg/mL | 1 mg/mL | 5 mL | (4) |
| L-ascorbic acid salt | 10 nM | .1 nM | 5 mL | (-20) |
| L-glutamine | 200 nM | 2 nM | 5 mL | (-20) |
| MCDB 201 Medium | 1X | .4X | 200 mL | (4) |
| Pen/strep | 100X | 1X | 5 mL | (-20) |
| PDGF | 10 ug/mL | 10 ng/mL | 500 uL | (-80) |
Filter at end.
