Difference between revisions of "Streptomycin"

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Inhibits protein synthesis; binds to 30S ribosomal subunit.
 
Inhibits protein synthesis; binds to 30S ribosomal subunit.
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The rpsL50 gene mutation conveys streptomycin resistance.  Resistance is recessive, so the presence of a wild type rpsL gene will convey sensitivity.  This allows counter-selection against the presence of the wt rpsL gene.  Mutations of wild type genes to convey resistance are common.
  
 
==Mechanism of Resistance==
 
==Mechanism of Resistance==
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==Usage Notes==
 
==Usage Notes==
  
#100 mg/mL stock solution in H<sub>2</sub>O.   
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#100 mg/mL stock solution in H<sub>2</sub>O.  <br> It is barely soluble in ethanol so you must dissolve it in H<sub>2</sub>O.
 
#Filter-sterilize with 0.22 &mu;m filter.   
 
#Filter-sterilize with 0.22 &mu;m filter.   
 
#Add 1 mL per liter of culture.
 
#Add 1 mL per liter of culture.
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J. Sambrook and D. W. Russell. ''Molecular Cloning: A Laboratory Manual.'' Cold Spring Harbor Laboratory Press, 3rd edition, 2001.
 
J. Sambrook and D. W. Russell. ''Molecular Cloning: A Laboratory Manual.'' Cold Spring Harbor Laboratory Press, 3rd edition, 2001.
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[[Category:Material]] [[Category:Antibiotic]]

Latest revision as of 09:48, 26 July 2007

Mode of Action

Inhibits protein synthesis; binds to 30S ribosomal subunit.

The rpsL50 gene mutation conveys streptomycin resistance. Resistance is recessive, so the presence of a wild type rpsL gene will convey sensitivity. This allows counter-selection against the presence of the wt rpsL gene. Mutations of wild type genes to convey resistance are common.

Mechanism of Resistance

Usage Notes

  1. 100 mg/mL stock solution in H2O.
    It is barely soluble in ethanol so you must dissolve it in H2O.
  2. Filter-sterilize with 0.22 μm filter.
  3. Add 1 mL per liter of culture.

References

J. H. Miller. A Short Course in Bacterial Genetics: A Laboratory Manual and Handbook for Escherichia coli and Related Bacteria. Cold Spring Harbor Laboratory Press, 1992.

J. Sambrook and D. W. Russell. Molecular Cloning: A Laboratory Manual. Cold Spring Harbor Laboratory Press, 3rd edition, 2001.