Difference between revisions of "Streptomyces:Other Bits/Antibiotic and Resistance Marker Information"

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===Antibiotic Concentrations and Usage===
 
===Antibiotic Concentrations and Usage===
  
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! style="width:175px;" | Name || style="width:150px;" | Stock Concentration (mg/ml) || style="width:150px;"| Final Concentration (&#956;g/ml) || style="width:100px;"| Usage in 10mL (&#956;L) || Dissolve in
 
! style="width:175px;" | Name || style="width:150px;" | Stock Concentration (mg/ml) || style="width:150px;"| Final Concentration (&#956;g/ml) || style="width:100px;"| Usage in 10mL (&#956;L) || Dissolve in
 
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! style="width:175px;" | E.coli Strain || style="width:150px;" | Plasmid / Cosmid || style="width:150px;"| E.coli Resistance Marker || style="width:100px;"| Plasmid Resistance Marker || Temperature Sensitive Replicon (ts)
 
! style="width:175px;" | E.coli Strain || style="width:150px;" | Plasmid / Cosmid || style="width:150px;"| E.coli Resistance Marker || style="width:100px;"| Plasmid Resistance Marker || Temperature Sensitive Replicon (ts)
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| BW25113 || pIJ790 (λRED) || None || cat || Replication @ 30C, Loss of λRED @ 37C
 
| BW25113 || pIJ790 (λRED) || None || cat || Replication @ 30C, Loss of λRED @ 37C
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| DH5α || None || N/A || ||
 
| DH5α || None || N/A || ||
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| DH5α || BT340 (FLP Recombinase) || None || cat / blacarb || Initial Replication @ 30C, Induction of FLP Recombinase & loss of plasmid @ 42C, Post recombination growth @37C
 
| DH5α || BT340 (FLP Recombinase) || None || cat / blacarb || Initial Replication @ 30C, Induction of FLP Recombinase & loss of plasmid @ 42C, Post recombination growth @37C
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| ET12567 [dam-13::Tn9] || pUZ8002 || cat || neo ||
 
| ET12567 [dam-13::Tn9] || pUZ8002 || cat || neo ||
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| || SuperCos 1 || || neo / blacarb ||  
 
| || SuperCos 1 || || neo / blacarb ||  
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| || pIJ773 || || acc(3)IV ||
 
| || pIJ773 || || acc(3)IV ||
 
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Revision as of 03:37, 27 July 2007

Media and Solutions - Antibiotic & Resistance Marker Information

Streptomyces @ UEA

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Antibiotic Concentrations and Usage

Name Stock Concentration (mg/ml) Final Concentration (μg/ml) Usage in 10mL (μL) Dissolve in
Ampicillin 100 100 10 sdH20
Apramycin 100 50 5 sdH20
Chloramphenicol 25 25 10 50%-100% EtOH
Hygromycin 50 25 5 sdH20
Kanamycin 100 50 5 sdH20
Nalidixic Acid 25 25 10 0.2M NaOH
Thiostrepton(e) 50 50 10 100% DMSO or 1:1 DMSO:EtOH
Viomycin 30 30 10 sdH20


E.coli Strain & Plasmid Resistance Markers

E.coli Strain Plasmid / Cosmid E.coli Resistance Marker Plasmid Resistance Marker Temperature Sensitive Replicon (ts)
BW25113 pIJ790 (λRED) None cat Replication @ 30C, Loss of λRED @ 37C
DH5α None N/A
DH5α BT340 (FLP Recombinase) None cat / blacarb Initial Replication @ 30C, Induction of FLP Recombinase & loss of plasmid @ 42C, Post recombination growth @37C
ET12567 [dam-13::Tn9] pUZ8002 cat neo
SuperCos 1 neo / blacarb
pIJ773 acc(3)IV