Difference between revisions of "Stephanopoulos:Projects"

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Our research is focused on Metabolic Engineering - the improvement of cellular
 
Our research is focused on Metabolic Engineering - the improvement of cellular
 
properties, using modern genetic tools. This field encompasses two important components:
 
properties, using modern genetic tools. This field encompasses two important components:

Revision as of 06:18, 12 July 2006

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<align=left><h1 style="color:maroon">Research</h1></align> <body bgcolor="#EEEEEE" marginheight="0" marginwidth="0" leftmargin="20" topmargin="0"

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Our research is focused on Metabolic Engineering - the improvement of cellular properties, using modern genetic tools. This field encompasses two important components: <b>a)</b> the modification of biochemical pathways inside cells and <b>b)</b> the rigorous evaluation of the resulting cellular phenotypes. In recent years our group has investigated the following topics:

               <ul>

<li><b><a href="Coryne.shtml">Aminoacid biosynthesis in

               <i>Corynebacterium glutamicum</i>.</b></a> 

<li><b><a href="Rhodo.shtml">Indene bioconversion in <i>Rhodococcus </i>Sp.</b></a>

<li><b><a href="Synecho.shtml">CO<sub>2</sub> fixation and product synthesis by the cyanobacterium <i>Synechocystis</i> Sp.</b></a>

               <li><b><a href="Diabetes.shtml">Elucidating type II diabetes by
               linking the expression and metabolic phenotypes of hepatoma and hepatocyte
               cells.</b></a>
               </ul>
               
           	To accomplish the above goals we make use of a diverse array of scientific tools and

methods, many of which have also become areas of research for our group:

               <ul>
               <p>
               <li>
               <b><a href="Bioinfo.shtml">Bioinformatics and Systems Biology</a></b> - Our group was

one of the first to realize the importance of computational tools for handling the large volume of data generated by microarrays and other technologies.

               <li>

<b><a href="Fluxes.shtml">Methods for intracellular flux determination</a></b>

               - Fluxes are determined by material balancing, NMR fine spectra analysis and

GC-MS measurements.

               <li>
               <b><a href="Microarray.shtml">DNA microarrays</a></b> - We have developed full 

genome microarrays for <i>Synechocystis</i> Sp., and partial microarrays for

               <i>C. glutamicum</i>, <i>E. coli</i>, and the mouse genomes. 
               <li>
               <b><a href="BioReactor.shtml">Bioreaction network analysis.</a></b>

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