Difference between revisions of "Stanford/BIOE44:Module 2"

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(Inventory for Arsenic Sensor)
(Inventory for Arsenic Sensor)
 
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|'''Volume'''
 
|'''Volume'''
 
|'''Concentration'''
 
|'''Concentration'''
 +
|-
 +
| Chris and Karina
 +
| pSB2k3 (digested (E and P), extracted, purified)
 +
| Ready for Ligation
 +
| 25 uL
 +
| 80 ng/uL
 
|-
 
|-
 
| Daniel and Aaditya
 
| Daniel and Aaditya
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| a substantial amount
 
| a substantial amount
 
| 203 ng/ul
 
| 203 ng/ul
 +
|-
 +
|
 +
| pSB2K3 vector (low copy vector and red-orange insert)
 +
| digested with E,P (for double ligation) and X,P (for single ligation); not running in gels yet
 +
| 25uL each
 +
| 273 ng/uL
 
|-
 
|-
 
|  
 
|  
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| 133 ng/uL
 
| 133 ng/uL
 
|-
 
|-
 +
|Sunny, Alex
 +
| color 002 purple insert
 +
| digested X,P, gel extracted
 +
|
 +
| 0 ng/uL (no DNA retrieved from gels)
 +
|-
 +
|
 +
|arsR insert
 +
|digested E,S, gel extracted
 
|
 
|
| MG1655 vector (low copy vector and red-orange insert)
+
|0 ng/uL (no DNA retrieved from gels)
| digested with E,P (for double ligation) and X,P (for single ligation); not running in gels yet
 
| 25uL each
 
| 273 ng/uL
 
 
|-
 
|-
 
|
 
|
|Sunny, Alex
 
 
| pSB2K3 vector (with K274111 insert)
 
| pSB2K3 vector (with K274111 insert)
 
| undigested
 
| undigested
 
| 48 uL
 
| 48 uL
 
| 8.8 ng/ul
 
| 8.8 ng/ul
|-
 
|
 
| color 002 purple insert
 
| digested X,P, gel extracted
 
|
 
| 0 ng/uL (no DNA retrieved from gels)
 
|-
 
|
 
| arsR insert
 
| digested E,S
 
|
 
| 11.1 ng/uL
 
 
|-
 
|-
 
|
 
|
 
| S03511 vector (low-medium copy #, inducible)
 
| S03511 vector (low-medium copy #, inducible)
 
| undigested
 
| undigested
| 48uL
+
| 0uL
 
| 41.2 ng/uL
 
| 41.2 ng/uL
 
|-
 
|-
 
|
 
|
| I3023 vector (low-medium copy #, inducible)
+
| J13023-1 vector (low-medium copy #, inducible)
 
| undigested
 
| undigested
| 48uL
+
| 27uL
 
| 97.2 ng/uL
 
| 97.2 ng/uL
 
|-
 
|-
 
|
 
|
| I3023 vector (low-medium copy #, inducible)
+
| J13023-2 vector (low-medium copy #, inducible)
 
| undigested
 
| undigested
| 48uL
+
| 27uL
 
| 93.7 ng/uL
 
| 93.7 ng/uL
 
|-
 
|-
 
|
 
|
| I3002 vector (low-medium copy #, constitutive)
+
| J13002-1 vector (low-medium copy #, constitutive)
 
| undigested
 
| undigested
| 48uL
+
| 28uL
 
| 103.9 ng/uL
 
| 103.9 ng/uL
 
|-
 
|-
 
|
 
|
| I3002 vector (low-medium copy #, constitutive)
+
| J13002-2 vector (low-medium copy #, constitutive)
 
| undigested
 
| undigested
| 48uL
+
| 29uL
 
| 109.1 ng/uL
 
| 109.1 ng/uL
 
|-
 
|-
|}
+
|Kosh & Isis
 
+
| Purple Color generator
{Sunny, Alex
+
| cut at XbaI & PstI, gel extracted (ready for ligation)
| pSB2K3 vector (with K274111 insert)
+
| ~5uL
| undigested
 
| 48 uL
 
| 8.8 ng/ul
 
|-
 
 
|  
 
|  
| color 002 purple insert
 
| digested X,P, gel extracted
 
|
 
| 0 ng/uL (no DNA retrieved from gels)
 
|-
 
|
 
| arsR insert
 
| digested E,S
 
|
 
| 11.1 ng/uL
 
 
|-
 
|-
 
|
 
|
| S03511 vector (low-medium copy #, inducible)
+
|Dark Green Color generator
| undigested
+
|cut at XbaI & PstI, gel extracted (ready for ligation)
| 48uL
+
|~5uL
| 41.2 ng/uL
+
|
 
|-
 
|-
 
|
 
|
| I3023 vector (low-medium copy #, inducible)
+
|Light Green Color generator
| undigested
+
|cut at XbaI & PstI, gel extracted (ready for ligation)
| 48uL
+
| ~5uL
| 97.2 ng/uL
 
|-
 
 
|
 
|
| I3023 vector (low-medium copy #, inducible)
 
| undigested
 
| 48uL
 
| 93.7 ng/uL
 
 
|-
 
|-
| I3002 vector (low-medium copy #, constitutive)
 
| undigested
 
| 48uL
 
| 103.9 ng/uL-
 
|
 
| -
 
| I3002 vector (low-medium copy #, constitutive)
 
| undigested
 
| 48uL
 
| 109.1 ng/uL-
 
 
|}
 
|}
 
==Introduction==
 
TBA
 
 
==Schedule==
 
 
TBA
 

Latest revision as of 12:33, 6 May 2010

Stanfordbioe44title2.jpg

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Module 2: Genetic Devices

Inventory for Arsenic Sensor

Group Members Part Status Volume Concentration
Chris and Karina pSB2k3 (digested (E and P), extracted, purified) Ready for Ligation 25 uL 80 ng/uL
Daniel and Aaditya arsR insert Digested at E and S 25 uL 36.8 ng/uL
arsR insert Digested at X and S, extracted from gel 2 gel bands (hasn't been purified yet)
Travis and Scott none
Frank, Claire, and Sanjay pSB2k3 vector (with K274111 insert) undigested a substantial amount 203 ng/ul
pSB2K3 vector (low copy vector and red-orange insert) digested with E,P (for double ligation) and X,P (for single ligation); not running in gels yet 25uL each 273 ng/uL
color 004 light green insert digested X,P and running in gels ~15uL 110 ng/uL
arsR insert digested E,S and running in gels ~15 uL 133 ng/uL
color 004 light green insert digested X,P and not running in gels yet 40 uL 110 ng/uL
arsR digested E,S and not running in gels yet 40 uL 133 ng/uL
Sunny, Alex color 002 purple insert digested X,P, gel extracted 0 ng/uL (no DNA retrieved from gels)
arsR insert digested E,S, gel extracted 0 ng/uL (no DNA retrieved from gels)
pSB2K3 vector (with K274111 insert) undigested 48 uL 8.8 ng/ul
S03511 vector (low-medium copy #, inducible) undigested 0uL 41.2 ng/uL
J13023-1 vector (low-medium copy #, inducible) undigested 27uL 97.2 ng/uL
J13023-2 vector (low-medium copy #, inducible) undigested 27uL 93.7 ng/uL
J13002-1 vector (low-medium copy #, constitutive) undigested 28uL 103.9 ng/uL
J13002-2 vector (low-medium copy #, constitutive) undigested 29uL 109.1 ng/uL
Kosh & Isis Purple Color generator cut at XbaI & PstI, gel extracted (ready for ligation) ~5uL
Dark Green Color generator cut at XbaI & PstI, gel extracted (ready for ligation) ~5uL
Light Green Color generator cut at XbaI & PstI, gel extracted (ready for ligation) ~5uL