Stanford/BIOE44:Module 2: Difference between revisions
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| Line 65: | Line 65: | ||
| 25uL each | | 25uL each | ||
| 273 ng/uL | | 273 ng/uL | ||
Sunny, Alex | Sunny, Alex | ||
| Line 103: | Line 102: | ||
| 93.7 ng/uL | | 93.7 ng/uL | ||
|- | |- | ||
| | |||
| I3002 vector (low-medium copy #, constitutive) | | I3002 vector (low-medium copy #, constitutive) | ||
| undigested | | undigested | ||
| 48uL | | 48uL | ||
| 103.9 ng/uL- | | 103.9 ng/uL | ||
|- | |||
| | | | ||
| I3002 vector (low-medium copy #, constitutive) | | I3002 vector (low-medium copy #, constitutive) | ||
| undigested | | undigested | ||
| 48uL | | 48uL | ||
| 109.1 ng/uL- | | 109.1 ng/uL | ||
| | |- | ||
|} | |||
==Introduction== | ==Introduction== | ||
Revision as of 16:08, 22 April 2010
Module 2: Genetic Devices
Inventory for Arsenic Sensor
| Group Members | Part | Status | Volume | Concentration | ||||
| Daniel and Aaditya | arsR insert | Digested at E and S | 25 uL | 36.8 ng/uL | ||||
| arsR insert | Digested at X and S, extracted from gel | 2 gel bands | (hasn't been purified yet) | |||||
| Travis and Scott | none | |||||||
| Frank, Claire, and Sanjay | pSB2k3 vector (with K274111 insert) | undigested | a substantial amount | 203 ng/ul | ||||
| color 004 light green insert | digested X,P and running in gels | ~15uL | 110 ng/uL | |||||
| arsR insert | digested E,S and running in gels | ~15 uL | 133 ng/uL | |||||
| color 004 light green insert | digested X,P and not running in gels yet | 40 uL | 110 ng/uL | |||||
| arsR | digested E,S and not running in gels yet | 40 uL | 133 ng/uL | |||||
| MG1655 vector (low copy vector and red-orange insert) | digested with E,P (for double ligation) and X,P (for single ligation); not running in gels yet | 25uL each | 273 ng/uL
Sunny, Alex |
pSB2K3 vector (with K274111 insert) | undigested | 48 uL | 8.8 ng/ul | |
| color 002 purple insert | digested X,P, gel extracted | 0 ng/uL (no DNA retrieved from gels) | ||||||
| arsR insert | digested E,S | 11.1 ng/uL | ||||||
| S03511 vector (low-medium copy #, inducible) | undigested | 48uL | 41.2 ng/uL | |||||
| I3023 vector (low-medium copy #, inducible) | undigested | 48uL | 97.2 ng/uL | |||||
| I3023 vector (low-medium copy #, inducible) | undigested | 48uL | 93.7 ng/uL | |||||
| I3002 vector (low-medium copy #, constitutive) | undigested | 48uL | 103.9 ng/uL | |||||
| I3002 vector (low-medium copy #, constitutive) | undigested | 48uL | 109.1 ng/uL |
Introduction
TBA
Schedule
TBA
