Difference between revisions of "Stanford/BIOE44:Module 2"

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(Inventory for Arsenic Sensor)
(Inventory for Arsenic Sensor)
Line 39: Line 39:
 
| color 004 light green insert
 
| color 004 light green insert
 
| digested X,P and running in gels
 
| digested X,P and running in gels
|  
+
| ~15uL
|  
+
| 110 ng/uL
 
|-
 
|-
 
|  
 
|  
 
| arsR insert
 
| arsR insert
 
| digested E,S and running in gels
 
| digested E,S and running in gels
|  
+
| ~15 uL
|
+
| 133 ng/uL
 
|-
 
|-
 
|
 
|
 
| color 004 light green insert
 
| color 004 light green insert
 
| digested X,P and not running in gels yet
 
| digested X,P and not running in gels yet
|
+
| 40 uL
|
+
| 110 ng/uL
 
|-
 
|-
 
|
 
|
 
| arsR
 
| arsR
 
| digested E,S and not running in gels yet
 
| digested E,S and not running in gels yet
|
+
| 40 uL
|
+
| 133 ng/uL
 
|-
 
|-
 
|
 
|
 
| MG1655 vector (low copy vector and red-orange insert)
 
| MG1655 vector (low copy vector and red-orange insert)
 
| digested with E,P (for double ligation) and X,P (for single ligation); not running in gels yet
 
| digested with E,P (for double ligation) and X,P (for single ligation); not running in gels yet
|
+
| 25uL each
|
+
| 273 ng/uL
 
|}
 
|}
  

Revision as of 15:51, 22 April 2010

Stanfordbioe44title2.jpg

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DNA Engineering        Devices        Synthesis        Baking        Testing

Module 2: Genetic Devices

Inventory for Arsenic Sensor

Group Members Part Status Volume Concentration
Daniel and Aaditya arsR insert Digested at E and S 25 uL 36.8 ng/uL
arsR insert Digested at X and S, extracted from gel 2 gel bands (hasn't been purified yet)
Travis and Scott none
Frank, Claire, and Sanjay MG1655 vector undigested a substantial amount 203 ng/ul
color 004 light green insert digested X,P and running in gels ~15uL 110 ng/uL
arsR insert digested E,S and running in gels ~15 uL 133 ng/uL
color 004 light green insert digested X,P and not running in gels yet 40 uL 110 ng/uL
arsR digested E,S and not running in gels yet 40 uL 133 ng/uL
MG1655 vector (low copy vector and red-orange insert) digested with E,P (for double ligation) and X,P (for single ligation); not running in gels yet 25uL each 273 ng/uL

Introduction

TBA

Schedule

TBA