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Mode of Action

Spectinomycin is a bacteriostatic agent that inhibits translocation of the peptidyl tRNA from the A site to the P site.

Working Concentrations and Stock Solutions

Up to 100mg/mL stock concentration. 100ug/mL final concentration.

Resistance Gene

Mutations in rpsE, the S5 protein, prevent spectinomycin from binding.

Inhibiting protein synthesis

In addition to being used for selection, spectinomycin can also be used to inhibit protein synthesis, for example, in the measurement of fluorescent protein maturation times. Some papers citing spectinomycin for ribosome interference include -

  1. pmid = 16408021 [Rackham]