SortoStat/Operation

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Revision as of 22:11, 28 April 2005 by Jason R. Kelly (talk | contribs)
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Steps

  1. Prepare lysis buffer by adding 10mg/ml Lysozyme to Pierce B-per Reagent.
  2. Spin down an overnight culture of cells at 2000rpm for 2 minutes to bring down large clumps of debris. The cells in the supernatant (which should still be cloudy) will serve as the cells to innoculate the device.