Difference between revisions of "SortoStat/Operation"

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lysis buffer:
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=Steps=
Pierce B-per Reagent and [[Lysozyme]]
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#Prepare lysis buffer by adding 10mg/ml [[Lysozyme]] to Pierce B-per Reagent.
 +
#Spin down an overnight culture of cells at 2000rpm for 2 minutes to bring down large clumps of debris.  The cells in the supernatant (which should still be cloudy) will serve as the cells to innoculate the device.

Revision as of 21:11, 28 April 2005

Steps

  1. Prepare lysis buffer by adding 10mg/ml Lysozyme to Pierce B-per Reagent.
  2. Spin down an overnight culture of cells at 2000rpm for 2 minutes to bring down large clumps of debris. The cells in the supernatant (which should still be cloudy) will serve as the cells to innoculate the device.