Smolke:Protocols/Insert prep: Difference between revisions
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**10 ul 10 uM Rev primer | **10 ul 10 uM Rev primer | ||
**250 ng plasmid | **250 ng plasmid | ||
**10 ul 1:10 Taq DNA polymerase (Starting | **10 ul 1:10 Taq DNA polymerase (Starting Mar 2013, we will use 5 ul of 1:20 Taq DNA polymerase)- YHW | ||
*After PCR, combine products from the five tubes, and run 5 ul on a gel to verify product length | *After PCR, combine products from the five tubes, and run 5 ul on a gel to verify product length |
Latest revision as of 23:11, 28 February 2013
Protocol
Notes
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