Smolke:Protocols/Freezer stock management and database: Difference between revisions
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*Determine number of stocks that can be made | *Determine number of stocks that can be made | ||
*Update freezer stock spreadsheet to determine stock position and print appropriate labels | *Update freezer stock spreadsheet to determine stock position and print appropriate labels | ||
*Harvest desired number of cells by spinning at 1400 rpm for | *Harvest desired number of cells by spinning at 1400 rpm for 5 min | ||
*Resuspend cells with heat-inactivated FBS + 10% DMSO to 10-20 million cells per ml | *Resuspend cells with heat-inactivated FBS + 10% DMSO to 10-20 million cells per ml | ||
*Aliquot cells into cryovials at 0.5 ml/vial (i.e., 5-10 million cells per vial) | *Aliquot cells into cryovials at 0.5 ml/vial (i.e., 5-10 million cells per vial) |
Latest revision as of 13:38, 7 December 2010
Freezer Stock DatabaseThe database is divided into two parts, the permanent archive and the working archive.
Labeling for All StocksAll freezer stocks in the database must have a cap insert and a printed label on the side of the vial. To create labels, use the template document in the Tissue Culture folder on the lab server. Print the labels using the Endy printer. Be sure to load the blank label sheet in the proper orientation. The labels of all freezer stocks must include the following information:
Lymphocyte (CTLL-2, Jurkat, and NK-92) Stocks
HEK Stocks
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