Difference between revisions of "Smolke:Protocols"

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:*[[Smolke:Protocols/Gel extraction|Gel extraction]]
 
:*[[Smolke:Protocols/Gel extraction|Gel extraction]]
 
:*[[Smolke:Protocols/Ligation|Ligation]]
 
:*[[Smolke:Protocols/Ligation|Ligation]]
:*[[Smolke:Protocols/Transformation|Transformation]]
+
:*[[Smolke:Protocols/Transformation|Electrocompetent Transformation]]
 +
:*[[Smolke:Protocols/Heat Shock Transformation|Heat Shock Transformation]]
 
:*[[Smolke:Protocols/Screening|Colony PCR]]
 
:*[[Smolke:Protocols/Screening|Colony PCR]]
 
:*[[Smolke:Protocols/Digest Screening|Screening by digestion]]
 
:*[[Smolke:Protocols/Digest Screening|Screening by digestion]]
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:*[[Smolke:Protocols/Freezer stocks|Making freezer stocks]]
 
:*[[Smolke:Protocols/Freezer stocks|Making freezer stocks]]
 
:*[[Smolke:Protocols/Gene assembly|Gene assembly]]
 
:*[[Smolke:Protocols/Gene assembly|Gene assembly]]
 +
:*[[Smolke:Protocols/EtBr stock|EtBr stock]]
  
 
===Yeast===
 
===Yeast===
 
:'''Protocols'''
 
:'''Protocols'''
 +
:*[[Smolke:Protocols/Yeast Media Recipes | Yeast Media Recipes]]
 
:*[[Smolke:Protocols/Dropout plates|Pouring yeast dropout plates]]
 
:*[[Smolke:Protocols/Dropout plates|Pouring yeast dropout plates]]
 
:*[[Smolke:Protocols/Yeast transformation|Yeast plasmid transformation]]
 
:*[[Smolke:Protocols/Yeast transformation|Yeast plasmid transformation]]
 +
:*[[Smolke:Protocols/Quick yeast transformation|Quick Yeast plasmid transformation]]
 
:*[[Smolke:Protocols/Yeast Colony PCR|Yeast colony PCR]]
 
:*[[Smolke:Protocols/Yeast Colony PCR|Yeast colony PCR]]
 
:*[[Smolke:Protocols/Western|Anti-V5 Western]]
 
:*[[Smolke:Protocols/Western|Anti-V5 Western]]
:*[[Smolke:Protocols/Library_transformation|Library-scale yeast transformations]]
+
:*[[Smolke:Protocols/Library_transformation|Library-scale yeast transformations & Gap repair]]
 
:*[[Smolke:Protocols/Chromosomal Modification|Yeast chromosomal modifications]]
 
:*[[Smolke:Protocols/Chromosomal Modification|Yeast chromosomal modifications]]
 
:*[[Smolke:Protocols/Yeast plasmid prep|Yeast plasmid prep]]
 
:*[[Smolke:Protocols/Yeast plasmid prep|Yeast plasmid prep]]
 +
:*[[Smolke:Protocols/Feeding yeast cultures|Feeding yeast cultures]]
 
:*[[Smolke:Protocols/Yeast metabolite extraction|Yeast metabolite extraction]]
 
:*[[Smolke:Protocols/Yeast metabolite extraction|Yeast metabolite extraction]]
 +
:*[[Smolke:Protocols/Heme measurements|Heme measurements]]
 
:'''Observations and Discussion'''
 
:'''Observations and Discussion'''
 
:*[http://wiki.yeastgenome.org/index.php/CommunityW303.html CSY3]
 
:*[http://wiki.yeastgenome.org/index.php/CommunityW303.html CSY3]
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===Tissue Culture room===
 
===Tissue Culture room===
 
:*[[Smolke:Protocols/Adherent cell care and feeding|Adherent cell care and feeding]]
 
:*[[Smolke:Protocols/Adherent cell care and feeding|Adherent cell care and feeding]]
 +
:*[[Smolke:Protocols/Media stocks|Media stocks]]
 
:*[[Smolke:Protocols/Freezer stock management and database|Freezer stock management and database]]
 
:*[[Smolke:Protocols/Freezer stock management and database|Freezer stock management and database]]
 
:*[[Smolke:Protocols/General Maintenance|General maintenance]]
 
:*[[Smolke:Protocols/General Maintenance|General maintenance]]
 +
:*[[Smolke:Protocols/General Sterility|General sterility]]
 +
 +
===Quanta===
 +
:*[[Smolke:Protocols/Preventing Clogs|Preventing Clogs]]
 +
:*[[Smolke:Protocols/Clearing Clogs|Clearing Clogs]]
 +
 +
===VYB===
 +
:*[[Smolke:Protocols/Guidelines|Guidelines]]
  
 
===Miscellaneous===
 
===Miscellaneous===
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:*[[Smolke:Protocols/Printing Posters|Poster Printing]]
 
:*[[Smolke:Protocols/Printing Posters|Poster Printing]]
 
:*[[Smolke:Protocols/Pipet-Aid|My pipet-aid is broken!]]
 
:*[[Smolke:Protocols/Pipet-Aid|My pipet-aid is broken!]]
:*[[Smolke:Protocols/Clearing Quanta Clogs|Clearing Quanta Clogs]]
 
 
:*[[Smolke:Protocols/Cleaning Bottles for the LC|Cleaning Bottles for the LC]]
 
:*[[Smolke:Protocols/Cleaning Bottles for the LC|Cleaning Bottles for the LC]]
  

Revision as of 11:30, 16 October 2012

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Note: If possible, please follow the standard protocol format.

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