Difference between revisions of "Smolke:Enzyme inventory"

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(Restriction Enzymes in Stocks)
(Restriction Enzymes in Stocks)
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! NdeI ||Y|| || ||  
 
! NdeI ||Y|| || ||  
 
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! NheI ||Y|| ||Y||
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! NheI || ||Y||Y||
 
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! NotI ||Y||Y|| ||Y  
 
! NotI ||Y||Y|| ||Y  

Revision as of 14:38, 30 November 2011

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Rules

  • Two restriction enzyme coolers are in -20C over the Quanta bay, one labeled common.
  • Only common enzymes may have backup, which are stored in main -20C.
  • Common enzymes: AgeI, AleI, ApaI, AvrII, BamHI, DpnI, EcoRI, EcoRV, HindIII, MfeI, NotI, SacI, SacII, SalI, XbaI, XhoI
  • If the enzyme is available in HF form, only the HF form will be in the backup unless otherwise requested.
  • When the working stock has been used up, replace with a new tube.
  • Backup ligases and CIP are also in the backup cooler in main -20C.

Restriction Enzymes in Stocks

Enzyme Regular HF Regular Backup HF Backup
AgeI Y Y
AleI Y Y
ApaI Y Y
AscI Y
AsiSI Y
BamHI Y Y Y
BglII Y
BsaI Y
BsrGI Y
BstBI Y
BstXI Y
Bsu36I Y
ClaI Y
DpnI Y Y
EcoRI Y Y
EcoRV Y Y
HindIII Y Y
KpnI Y Y
MfeI Y Y
NdeI Y
NheI Y Y
NotI Y Y Y
PacI Y
PmeI Y
PvuII Y
SacI Y Y
SacII Y Y
SaII Y Y Y
SapI Y
SpeI Y
SphI Y
SwaI Y
XbaI Y Y
XhoI Y Y
XmaI Y

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