Difference between revisions of "Skatebro:Pouring Plates"

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[[Skatebro:Project08]]
 
  
==Tips==
 
*Don't forget to label
 
*Doublecheck that antibiotics are in stock
 
 
==Steps==
 
#Set water bath to 50 degrees
 
#Obtain 500 ml 1.2% LB agar from the media room
 
#Melt LB in microwave:
 
#*loosen the cap a lot, lay down paper towels in case of spill, use 50% power (enter time, press Power, 5, Start)
 
#*monitor as you melt, takes approx. 10 minutes per bottle (if doing 2 or 3 bottles simultaneously, add 5 minutes)
 
#Cool in waterbath to 50-60 degrees
 
#Add antibiotics and swirl: (for 500mL of LB Agar)
 
#*500μL Amp stock (Amp stock = 50mg/ml)
 
#*1mL Kan stock (Kan stock = 10mg/ml)
 
#*750μL Gent stock
 
#*500μL Tet stock
 
#*500μL Chl stock
 
#Pour, let stand, label, store in fridge
 

Latest revision as of 03:07, 9 February 2008