Difference between revisions of "Size selective DNA precipitation"
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Revision as of 14:36, 27 August 2008
Kersten S. Rabe
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A very fast and easy method for the size-selective removal of smaller DNA from larger fragments. By adjusting the PEG and MgCl2 concentration the range of precipitated DNA fragments can be adjusted.
- DNA to be separated
- 30% (w/v) PEG 8000/30 mM MgCl2
- TE Buffer, pH 8.0 (10 mM TRIS-HCl, 1 mM EDTA, pH 8.0)
- Centrifuge which can to upto 10.000 rcf (=g)
- Appropriate tubes for the centrifuge
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