Difference between revisions of "Silver: 50x TAE"

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50x TAE is 2M Tris, 2M Acetic Acid (glacial), and 50mM EDTA in its final 1L volume.
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The final concentrations of reagents in 50x TAE is 2M Tris, 2M Acetic Acid, and 50mM EDTA.
  
 
Per 1L:
 
Per 1L:
  
 
*242g Tris base
 
*242g Tris base
*57.1 ml acetic acid
+
*57.1 ml acetic acid (glacial)
 
*100ml 0.5 M EDTA
 
*100ml 0.5 M EDTA
  
 
'''DO NOT AUTOCLAVE'''
 
'''DO NOT AUTOCLAVE'''
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 +
[[Category:Material]]
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[[Category:Buffers]]
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[[Category:Agarose gel electrophoresis]]

Latest revision as of 16:42, 20 March 2013

The final concentrations of reagents in 50x TAE is 2M Tris, 2M Acetic Acid, and 50mM EDTA.

Per 1L:

  • 242g Tris base
  • 57.1 ml acetic acid (glacial)
  • 100ml 0.5 M EDTA

DO NOT AUTOCLAVE