Difference between revisions of "Shreffler:Notebook/retinoic acid trans signaling/2009/07/30"

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(Autocreate 2009/07/30 Entry for Shreffler:Notebook/retinoic_acid_trans_signaling)
 
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==Entry title==
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=='''[[User:Neisha A. Rivers|Neisha A. Rivers]]'''==
* Insert content here...
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Measuring Luciferase
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* Transfer cells to 1.5 ml eppendorfs
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* Spin cells down for 500g for 10 min
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* Aspirate supernatants, then added 125 ul of PLB buffer, vortex
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* Allow to shake at room temperature for 30 min
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* Spin cells at 1400g for 2 min, leave supernatants
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* Add 25 ul of substrate (LAR) and 5 ul of lysate (supernatants)
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Results: Luciferase was not detected in any of the samples
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Next Steps: Beta-gal assay, and a different transfection method - Lipofectamine
  
  

Revision as of 10:52, 30 July 2009

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Neisha A. Rivers

Measuring Luciferase


  • Transfer cells to 1.5 ml eppendorfs
  • Spin cells down for 500g for 10 min
  • Aspirate supernatants, then added 125 ul of PLB buffer, vortex
  • Allow to shake at room temperature for 30 min
  • Spin cells at 1400g for 2 min, leave supernatants
  • Add 25 ul of substrate (LAR) and 5 ul of lysate (supernatants)

Results: Luciferase was not detected in any of the samples

Next Steps: Beta-gal assay, and a different transfection method - Lipofectamine