Difference between revisions of "Sean Lauber:Using the Kras G12D Mouse Model for Lung Cancer"

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(New page: This procedure involves first precipitating adenovirus before it is administered by intubation. For 1000 ul: CaCl2 2 ul 10X EMEM 100 ul 1. Calculate the appr...)
 
 
Line 2: Line 2:
  
 
   For 1000 ul:
 
   For 1000 ul:
     CaCl2        2 ul
+
     2 M CaCl2        2 ul
     10X EMEM      100 ul
+
     10X EMEM         100 ul
      
+
    0.2 M L-glut     10 ul
 +
    Virus            x ul
 +
     Sterile water    888-x ul 
 +
   
  
  
1. Calculate the appropriate amount of virus to use
+
1. Calculate the appropriate amount of virus and add it (without CaCl2) along with the other reagents to an ependorff
  
2.
+
2. 20 minutes before you plan on intubating, add the CaCl2 and vortex
 +
 
 +
3. Use within 1 hour (after 20 minutes of waiting)

Latest revision as of 11:49, 18 July 2013

This procedure involves first precipitating adenovirus before it is administered by intubation.

 For 1000 ul:
   2 M CaCl2         2 ul
   10X EMEM          100 ul
   0.2 M L-glut      10 ul 
   Virus             x ul
   Sterile water     888-x ul  
    


1. Calculate the appropriate amount of virus and add it (without CaCl2) along with the other reagents to an ependorff

2. 20 minutes before you plan on intubating, add the CaCl2 and vortex

3. Use within 1 hour (after 20 minutes of waiting)