Difference between revisions of "Sean Lauber:Adenovirus infection (in vitro)"

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3. Wash cells with 100 ul of PBS two times.
 
3. Wash cells with 100 ul of PBS two times.
  
4. Add 10 pfu of the virus in 10 ul of PBS on top of the cells.
+
4. Add 10 pfu/cell (MOI) of the virus in 10 ul of PBS on top of the cells.
  
 
5. Incubate at 37°C for 30 minutes.
 
5. Incubate at 37°C for 30 minutes.

Revision as of 07:59, 25 January 2013

This procedure is optimized for using 96-well plates (0.32 cm^2).

1. Seed 2000 cells per well

2. Incubate overnight at 37°C/5% CO2.

3. Wash cells with 100 ul of PBS two times.

4. Add 10 pfu/cell (MOI) of the virus in 10 ul of PBS on top of the cells.

5. Incubate at 37°C for 30 minutes.

6. Add 100 ul of media on top of the cells (don't remove the virus)

7. Incubate for as long as you need at 37°C/5% CO2


Possible to go from 20-50 pfu as well, although 10 seems to work just fine.