Schwekendiek Lab Inventory
From OpenWetWare
Restriction Enzymes
| Item | Fast Digest | Supplier | Availability | Location | Last update |
|---|---|---|---|---|---|
| Acc65I (KpnI) | yes | Fermentas | in stock | Freezer 1 | --Axel 21:08, 24 January 2007 (EST) |
| BamH1 | yes | Fermentas | in stock | Freezer 1 | --Axel 16:34, 18 March 2008 (CDT) |
| BglII | yes | Fermentas | in stock | Freezer 1 | --Axel 16:34, 18 March 2008 (CDT) |
| Cla1 | yes | Fermentas | in stock | Freezer 1 | --Axel 16:34, 18 March 2008 (CDT) |
| EcoR1 | yes | Fermentas | in stock | Freezer 1 | --Axel 16:34, 18 March 2008 (CDT) |
| EcoRV | yes | Fermentas | in stock | Freezer 1 | --Axel 16:34, 18 March 2008 (CDT) |
| HindIII | yes | Fermentas | in stock | Freezer 1 | --Axel 16:34, 18 March 2008 (CDT) |
| Kpn1 | yes | Fermentas | in stock | Freezer 1 | --Axel 16:34, 18 March 2008 (CDT) |
| Nco1 | yes | Fermentas | in stock | Freezer 1 | --Axel 16:34, 18 March 2008 (CDT) |
| Not1 | yes | Fermentas | in stock | Freezer 1 | --Axel 16:34, 18 March 2008 (CDT) |
| Pst1 | yes | Fermentas | in stock | Freezer 1 | --Axel 16:34, 18 March 2008 (CDT) |
| Sac1 | yes | Fermentas | in stock | Freezer 1 | --Axel 16:34, 18 March 2008 (CDT) |
| Sal1 | no | Fermentas | in stock | Freezer 1 | --Axel 16:34, 18 March 2008 (CDT) |
| Sma1 | yes | Fermentas | in stock | Freezer 1 | --Axel 16:34, 18 March 2008 (CDT) |
| SpeI (BcuI) | yes | Fermentas | in stock | Freezer 1 | --Axel 16:34, 18 March 2008 (CDT) |
| Xba1 | yes | Fermentas | in stock | Freezer 1 | --Axel 16:34, 18 March 2008 (CDT) |
| Xho1 | yes | Fermentas | in stock | Freezer 1 | --Axel 16:34, 18 March 2008 (CDT) |
DNA Modifying Enzymes
| Enzyme | Supplier | Order No. | Availability | Location | Last update |
|---|---|---|---|---|---|
| Taq DNA Polymerase | Fermentas | N/A | in stock | Freezer 1 | --Axel 16:35, 18 March 2008 (CDT) |
| Taq DNA Polymerase | Invitrogen | N/A | in stock | Freezer 1 | --Axel 16:25, 18 March 2008 (CDT) |
| T4-DNA Ligase | Fermentas | N/A | in stock | Freezer 1 | --Axel 16:35, 18 March 2008 (CDT) |
| DNAse I | Fermentas | N/A | in stock | Freezer 1 | --Axel 16:35, 18 March 2008 (CDT) |
| M-MuLV Reverse Transcriptase | Fermentas | N/A | in stock | Freezer 1 | --Axel 16:35, 18 March 2008 (CDT) |
Bacterial Strains
| Strain | Resistance | Source | Last update |
|---|---|---|---|
| EHA101 | KAN | Dr. Walter | --Axel 16:24, 18 March 2008 (CDT) |
| LBA4404 | Dr. Matthews | --Axel 16:24, 18 March 2008 (CDT) | |
| AGLO | Dr. Abebe | --Axel 16:24, 18 March 2008 (CDT) | |
| DH5-alpha | None | Dr. Abebe | --Axel 21:08, 24 January 2007 (EST) |
| DB3.1 | None | Dr. Lizarazo (MIT) | --Axel 21:08, 24 January 2007 (EST) |
| Transbacter | Cambia (considered) | --Axel 21:08, 24 January 2007 (EST) |
Antibiotic Stocks
| Antibiotic | Concentration | Location | Supplier | Order No. | Last update |
|---|---|---|---|---|---|
| Ampicillin | 60 mg/ml | Freezer 1 | Gold Biotech | --Axel 16:43, 18 March 2008 (CDT) | |
| Kanamycin | 50 mg/ml | Freezer 1 | Gold Biotech | --Axel 16:43, 18 March 2008 (CDT) | |
| Chloramphenicol | 34 mg/ml | Freezer 1 | Gold Biotech | --Axel 16:43, 18 March 2008 (CDT) | |
| Spectomycin | Freezer 1 | Gold Biotech | --Axel 16:43, 18 March 2008 (CDT) | ||
| Rifampicin | Freezer 1 | Gold Biotech | --Axel 16:43, 18 March 2008 (CDT) | ||
| Carbenicillin | Freezer 1 | Gold Biotech | --Axel 16:43, 18 March 2008 (CDT) |
Pasmids
| Plasmid | Map | Source | Last update |
|---|---|---|---|
| pBluescript KS | N/A | Stratagene | --Axel 21:08, 24 January 2007 (EST) |
| P-GFP | N/A | Dr. Abebe (UNI) | --Axel 21:08, 24 January 2007 (EST) |
| pSB1A3 | N/A | Dr. Lizarazo (MIT) | --Axel 21:08, 24 January 2007 (EST) |
| pSB1A7 | N/A | Dr. Lizarazo (MIT) | --Axel 21:08, 24 January 2007 (EST) |
| pSB1A10 | N/A | Dr. Lizarazo (MIT) | --Axel 21:08, 24 January 2007 (EST) |
| J52017 | N/A | Dr. Lizarazo (MIT) | --Axel 21:08, 24 January 2007 (EST) |
| J61002 | N/A | Dr. Lizarazo (MIT) | --Axel 21:08, 24 January 2007 (EST) |
| J61003 | N/A | Dr. Lizarazo (MIT) | --Axel 21:08, 24 January 2007 (EST) |
| pCAMBIA0380 | N/A | CAMBIA | --Axel 21:08, 24 January 2007 (EST) |
| pCAMBIA0390 | N/A | CAMBIA | --Axel 21:08, 24 January 2007 (EST) |
| pCAMBIA1200 | N/A | CAMBIA | --Axel 21:08, 24 January 2007 (EST) |
| pCAMBIA1201 | N/A | CAMBIA | --Axel 21:08, 24 January 2007 (EST) |
| pCAMBIA1281Z | N/A | CAMBIA | --Axel 21:08, 24 January 2007 (EST) |
| pCAMBIA1291Z | N/A | CAMBIA | --Axel 21:08, 24 January 2007 (EST) |
| pCAMBIA1300 | N/A | CAMBIA | --Axel 21:08, 24 January 2007 (EST) |
| pCAMBIA1301 | N/A | CAMBIA | --Axel 21:08, 24 January 2007 (EST) |
| pCAMBIA1302 | N/A | CAMBIA | --Axel 21:08, 24 January 2007 (EST) |
| pCAMBIA1303 | N/A | CAMBIA | --Axel 21:08, 24 January 2007 (EST) |
| pCAMBIA1304 | N/A | CAMBIA | --Axel 21:08, 24 January 2007 (EST) |
| pCAMBIA1380 | N/A | CAMBIA | --Axel 21:08, 24 January 2007 (EST) |
| pCAMBIA1381Xa | N/A | CAMBIA | --Axel 21:08, 24 January 2007 (EST) |
| pCAMBIA1381Xb | N/A | CAMBIA | --Axel 21:08, 24 January 2007 (EST) |
| pCAMBIA1381Xc | N/A | CAMBIA | --Axel 21:08, 24 January 2007 (EST) |
| pCAMBIA1381Z | N/A | CAMBIA | --Axel 21:08, 24 January 2007 (EST) |
| pCAMBIA1390 | N/A | CAMBIA | --Axel 21:08, 24 January 2007 (EST) |
| pCAMBIA1391 | N/A | CAMBIA | --Axel 21:08, 24 January 2007 (EST) |
| pCAMBIA1391Xa | N/A | CAMBIA | --Axel 21:08, 24 January 2007 (EST) |
| pCAMBIA1391Xb | N/A | CAMBIA | --Axel 21:08, 24 January 2007 (EST) |
| pCAMBIA1391Xc | N/A | CAMBIA | --Axel 21:08, 24 January 2007 (EST) |
| pCAMBIA1391Z | N/A | CAMBIA | --Axel 21:08, 24 January 2007 (EST) |
| pCAMBIA2200 | N/A | CAMBIA | --Axel 21:08, 24 January 2007 (EST) |
| pCAMBIA2201 | N/A | CAMBIA | --Axel 21:08, 24 January 2007 (EST) |
| pCAMBIA2300 | N/A | CAMBIA | --Axel 21:08, 24 January 2007 (EST) |
| pCAMBIA2301 | N/A | CAMBIA | --Axel 21:08, 24 January 2007 (EST) |
| pCAMBIA1105.1 | N/A | CAMBIA | --Axel 21:08, 24 January 2007 (EST) |
| pCAMBIA1305.1 | N/A | CAMBIA | --Axel 21:08, 24 January 2007 (EST) |
| pCAMBIA1305.2 | N/A | CAMBIA | --Axel 21:08, 24 January 2007 (EST) |
| pCAMBIA0305.1 | N/A | CAMBIA | --Axel 21:08, 24 January 2007 (EST) |
| pCAMBIA0305.2 | N/A | CAMBIA | --Axel 21:08, 24 January 2007 (EST) |
Electrophoresis
| Item | Supplier | Availability | Last update |
|---|---|---|---|
| Top Vision LE GQ Agarose | Fermentas | in stock | --Axel 21:08, 24 January 2007 (EST) |
| 6x SDS Loading Dye | Fermentas | in stock | --Axel 21:08, 24 January 2007 (EST) |
| 6x Mass Ruler Loading Dye | Fermentas | in stock | --Axel 21:08, 24 January 2007 (EST) |
| 6x Orange Dye | Fermentas | in stock | --Axel 21:08, 24 January 2007 (EST) |
| Fast Ruler low range ladder | Fermentas | in stock | --Axel 21:08, 24 January 2007 (EST) |
| 1 kb ladder | Fermentas | in stock | --Axel 21:08, 24 January 2007 (EST) |
| O´Gene Ruler 100 bp plus | Fermentas | in stock | --Axel 21:08, 24 January 2007 (EST) |
| 2x Loading Dye for RNA | Fermentas | in stock | --Axel 21:08, 24 January 2007 (EST) |
| RiboRuler RNA ladder low range | Fermentas | in stock | --Axel 21:08, 24 January 2007 (EST) |
| RiboRuler RNA ladder high range | Fermentas | in stock | --Axel 21:08, 24 January 2007 (EST) |
Buffers
| Item | Availability | Last update |
|---|---|---|
| 50x TAE | in stock | --Axel 21:08, 24 January 2007 (EST) |
| 10x TBE | in stock | --Axel 21:08, 24 January 2007 (EST) |
Labelling & Detection
| Item | Supplier | Order No. | Last update |
|---|---|---|---|
| Biotin Chromogenic Detection Kit | Fermentas | --Axel 21:08, 24 January 2007 (EST) | |
| DecaLabel DNA Labelling Kit | Fermentas | --Axel 21:08, 24 January 2007 (EST) | |
| SensiBlot Plus Nylon Membrane | Fermentas | --Axel 21:08, 24 January 2007 (EST) |
Plant Tissue Culture Media
| Item | Supplier | Order No. | Availability | Last update |
|---|---|---|---|---|
| B5 vitamins | Caisson Labs | in stock | --Axel 21:08, 24 January 2007 (EST) |
Plant Hormone Stocks
| Hormone | Concentration | Supplier | Order No. | Last update |
|---|---|---|---|---|
| TDZ | Caisson Labs | --Axel 21:08, 24 January 2007 (EST) | ||
| IAA | N/A | --Axel 21:08, 24 January 2007 (EST) | ||
| NAA | N/A | --Axel 16:21, 18 March 2008 (CDT) | ||
| 2,4-D | N/A | --Axel 16:21, 18 March 2008 (CDT) | ||
| GA3 | N/A | --Axel 16:21, 18 March 2008 (CDT) |
Sterile Plant Cultures
| Plant | Genotype/Cultivar/Variety | Status | Last update |
|---|---|---|---|
| Humulus lupulus | Tettnanger | established | --Axel 21:08, 29 May 2007 (EST) |
Chemicals
| Item | Supplier | Order No. | Availability | Last update |
|---|---|---|---|---|
| CTAB | N/A | in stock | --Axel 16:13, 18 March 2008 (CDT) | |
| Chloroform/Isoamylalcohol | Fisher Scientific | in stock | --Axel 16:13, 18 March 2008 (CDT) | |
| DMSO | N/A | in stock | --Axel 16:09, 18 March 2008 (CDT) | |
| Ethidium bromide | Fisher Scientific | in stock | --Axel 16:13, 18 March 2008 (CDT) | |
| IPTG | N/A | in stock | --Axel 16:13, 18 March 2008 (CDT) | |
| PEG 8000 | Fisher Scientific | in stock | --Axel 16:13, 18 March 2008 (CDT) | |
| Phenol/TE | Fisher Scientific | in stock | --Axel 16:13, 18 March 2008 (CDT) | |
| PVP | N/A | in stock | --Axel 16:13, 18 March 2008 (CDT) | |
| X-Gal | N/A | in stock | --Axel 16:13, 18 March 2008 (CDT) |
Instrumentation
| Item | Availability | Last update |
|---|---|---|
| Gradient PCR Cycler | 2 | --Axel 21:08, 24 January 2007 (EST) |
| Laminar Flow Hood | 2 | --Axel 21:08, 24 January 2007 (EST) |
| Chemical Hood | 1 | --Axel 21:08, 24 January 2007 (EST) |
| Plant tissue culture incubators | 3 | --Axel 21:08, 24 January 2007 (EST) |
| Temporary Immersion System | 40 | --Axel 21:08, 24 January 2007 (EST) |
| Gel electrophoresis | 4 | --Axel 16:18, 18 March 2008 (CDT) |
| Power supplies | 2 | --Axel 21:08, 24 January 2007 (EST) |
| Bench-top centrifuge | 1 | --Axel 21:08, 24 January 2007 (EST) |
| Shaker Incubator with light bench | 1 | --Axel 21:08, 24 January 2007 (EST) |
| Balance 0.01g | 1 | --Axel 21:08, 24 January 2007 (EST) |
| Balance 0.001g | 1 | --Axel 21:08, 24 January 2007 (EST) |
| Gyro Twister | 1 | --Axel 16:18, 18 March 2008 (CDT) |
| Gyro Shaker | 1 | --Axel 16:18, 18 March 2008 (CDT) |
| pH-meter | 1 | --Axel 21:08, 24 January 2007 (EST) |
| -20 Freezer | 2 | --Axel 16:37, 18 March 2008 (CDT) |
| -80 Freezer | 1 | --Axel 21:08, 24 January 2007 (EST) |
