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Here we will post protocols that people in the lab generally use. Many of these will be lab-specific protocols, but if you think the protocol you are posting is more generally applicable, please link it to the general Protocols page. Also, if you typically do a variation of the protocol that is posted, please list it on the same page as a separate, alternative protocol. If possible, also try to note why what you do is different.

PLEASE NOTE: Blindly following a protocol is not a good substitute for actually talking to someone who has done the experiment. When posting protocols, please also include the name(s) of people in the lab who will be good to talk to about the protocol.

Sauer:ClpX purification

Sauer:ClpP purification

Sauer:In vitro degradation of GFP-ssrA by ClpXP

Sauer:Expression/purification of 35S-Met proteins

Sauer:In vitro degradation of radiolabeled proteins by ClpXP

Sauer:ATPase assays

Sauer:Western blot

Sauer:Quantitative Western blot

Sauer:Site-directed mutagenesis

Sauer:Competent cell preparation

Sauer:P1vir phage transduction

Sauer:Northern blot

Sauer:Silver staining

Sauer:In vivo tmRNA tagging assays

Sauer:In vitro transcription with T7 RNA polymerase

Sauer:Phenol/chloroform extraction

Sauer:bis-Tris SDS-PAGE, the very best

Sauer:Nucleic acid quantitation