Sauer:Protocols

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Protein Expression/Purification Sauer:ClpX purification Sauer:His6 ClpX purification Sauer:ClpP purification Sauer:ClpA purification Sauer:SspB purification Sauer:Expression/purification of 35S-Met proteins Sauer:Purification of His6-tagged proteins Sauer:Lysing E. coli with Lysozymes Sauer:GFP-H6-ssrA purification Sauer:GST-sFtsH purification In vitro enzymatic assays Sauer:In vitro degradation of GFP-ssrA by ClpXP Sauer:In vitro degradation of GFP-ssrA by ClpAP Sauer:In vitro degradation of radiolabeled proteins by ClpXP PROTOCOL NEEDED! Sauer:ATPase assays Sauer:In vitro peptide degradation by ClpXP Proteins: in vitro Sauer:Silver staining Sauer:bis-Tris SDS-PAGE, the very best Nucleic acids: in vitro Sauer:In vitro transcription with T7 RNA polymerase Sauer:Phenol/chloroform extraction Quantitating nucleic acids Sauer:Site-directed mutagenesis Sauer:RNA Purification from E. coli Sauer:Stitching Genes by PCR

Analysis of proteins or RNA in vivo Sauer:Western blot Sauer:Quantitative Western blot Sauer:Northern blot PROTOCOL NEEDED! Sauer:In vivo tmRNA tagging assays PROTOCOL NEEDED! Sauer:beta-Galactosidase Assay (A better Miller) More in vivo goodies Sauer:Competent cell preparation PROTOCOL NEEDED! Sauer:P1vir phage transduction Sauer:Plasmid-born λRed recombineering using dsDNA Other good stuff Generation of genomic libraries Sauer:HPLC protocol Sauer:FPLC protocol