Sauer:Protocols: Difference between revisions
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[[Sauer:In vitro degradation of radiolabeled proteins by ClpXP]] PROTOCOL NEEDED! | [[Sauer:In vitro degradation of radiolabeled proteins by ClpXP]] PROTOCOL NEEDED! | ||
[[Sauer:ATPase assays]] | [[Sauer:ATPase assays]] | ||
===Proteins: ''in vitro''=== | ===Proteins: ''in vitro''=== |
Revision as of 20:29, 27 November 2006
Please link protocols to the general Protocols page if appropriate.
Protein Expression/PurificationSauer:Expression/purification of 35S-Met proteins Sauer:Purification of His6-tagged proteins In vitro enzymatic assaysSauer:In vitro degradation of GFP-ssrA by ClpXP Sauer:In vitro degradation of radiolabeled proteins by ClpXP PROTOCOL NEEDED! Proteins: in vitroSauer:bis-Tris SDS-PAGE, the very best Nucleic acids: in vitroSauer:In vitro transcription with T7 RNA polymerase Sauer:Phenol/chloroform extraction Sauer:Nucleic acid quantitation Sauer:Site-directed mutagenesis
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Analysis of proteins or RNA in vivoSauer:Western blot PROTOCOL NEEDED! Sauer:Quantitative Western blot PROTOCOL NEEDED! Sauer:Northern blot PROTOCOL NEEDED! Sauer:In vivo tmRNA tagging assays PROTOCOL NEEDED! Sauer:beta-Galactosidase Assay (A better Miller) More in vivo goodiesSauer:Competent cell preparation PROTOCOL NEEDED! Sauer:P1vir phage transduction Other good stuff |