Sack and Yarov-Yarovoy:Protocols: Difference between revisions
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==Cell Culture== | ==Cell Culture== | ||
[[Sack: Cell Media with Selection Agents (Blasticidin, Zeocin, Geneticin)|Cell Media with Selection Agents (Blasticidin, Zeocin, Geneticin) | [[Sack: Cell Media with Selection Agents (Blasticidin, Zeocin, Geneticin)|Cell Media with Selection Agents (Blasticidin, Zeocin, Geneticin)]] [http://openwetware.org/images/8/84/Cell_Media_with_Gen_Selection_Agents_4-5-2012.pdf .]<br> | ||
[[Sack: Cell Media with Selection Agents (Blasticidin, Zeocin)|Cell Media with Selection Agents (Blasticidin, Zeocin) | [[Sack: Cell Media with Selection Agents (Blasticidin, Zeocin)|Cell Media with Selection Agents (Blasticidin, Zeocin)]] [http://openwetware.org/images/e/e5/Cell_Media_with_Selection_Agents_11-15-2011KE.pdf .]<br> | ||
[[Sack: Cell Splitting Protocol|Cell Splitting Protocol | [[Sack: Cell Splitting Protocol|Cell Splitting Protocol]][http://openwetware.org/images/a/ac/Cell_Splitting_Protocol.pdf .]<br> | ||
[[Sack: Cell Transfection (Lipofectamine LTX)|Cell Transfection (Lipofectamine LTX) | [[Sack: Cell Transfection (Lipofectamine LTX)|Cell Transfection (Lipofectamine LTX)]][http://openwetware.org/images/e/e4/Cell_Transfection_%28Lipofectamine_LTX%29_5-01-2012_KE.pdf .]<br> | ||
[[Sack: Cell Transfection (Lipofectamine)|Cell Transfection (Lipofectamine) | [[Sack: Cell Transfection (Lipofectamine)|Cell Transfection (Lipofectamine)]][http://openwetware.org/images/3/37/Cell_Transfection_%28Lipofectamine%29_8-14-2012_AS.pdf .]<BR> | ||
[[Sack: Cell Transfection (Novachoice)|Cell Transfection (Novachoice) | [[Sack: Cell Transfection (Novachoice)|Cell Transfection (Novachoice)]][http://openwetware.org/images/6/61/Cell_Transfection_%28Novachoice%29_5-04-2012_KE.pdf .]<BR> | ||
[[Sack: Cell Harvesting for Electrophysiology|Cell Harvesting for Electrophysiology | [[Sack: Cell Harvesting for Electrophysiology|Cell Harvesting for Electrophysiology]][http://openwetware.org/images/4/48/Cell_Harvesting_for_Electrophysiology_7-18-2012_KE.pdf .]<br> | ||
[[Sack: Cell Plating for Electrophysiology|Cell Plating for Electrophysiology | [[Sack: Cell Plating for Electrophysiology|Cell Plating for Electrophysiology]] [http://openwetware.org/images/1/1c/Cell_Plating_for_Electrophysiology_SackLab.pdf .]<br> | ||
[[Sack: Freezing CHO cells|Freezing CHO Cells | [[Sack: Freezing CHO cells|Freezing CHO Cells]][http://openwetware.org/images/5/52/Freezing_CHOcells_SackLab.pdf .]<BR> | ||
[[Sack: Plating Subclones for Ephys|Plating Subclones for Ephys | [[Sack: Plating Subclones for Ephys|Plating Subclones for Ephys]][http://openwetware.org/images/d/dc/Plating_Subclones_for_Ephys_SackLab.pdf .]<BR> | ||
[[Sack: Plating Subclones for Flourescent Screening|Plating Subclones for Fluorescent Screening | [[Sack: Plating Subclones for Flourescent Screening|Plating Subclones for Fluorescent Screening]][http://openwetware.org/images/2/21/Plating_subclones_for_fluorescent_screening_SackLab.pdf .]<BR> | ||
[[Sack: Subcloning Cells| Subcloning Cells | [[Sack: Subcloning Cells| Subcloning Cells]][http://openwetware.org/images/4/4a/Subcloning_Cells_SackLab_2-23-2011_KE.pdf .]<BR> | ||
[[Sack: Testing TREX Subclones Ephys| Testing TREX Subclones Ephys | [[Sack: Testing TREX Subclones Ephys| Testing TREX Subclones Ephys]][http://openwetware.org/images/9/90/Testing_TREX_Subclones_Ephys.pdf .]<BR> | ||
[[Sack: Thawing CHOcells| Thawing CHO cells | [[Sack: Thawing CHOcells| Thawing CHO cells][http://openwetware.org/images/0/01/Thawing_CHOcells_SackLab_7-9-2012.pdf .]<BR> | ||
==electrophysiology== | ==electrophysiology== | ||
Revision as of 12:06, 25 October 2012
Cell Culture
Cell Media with Selection Agents (Blasticidin, Zeocin, Geneticin) .
Cell Media with Selection Agents (Blasticidin, Zeocin) .
Cell Splitting Protocol.
Cell Transfection (Lipofectamine LTX).
Cell Transfection (Lipofectamine).
Cell Transfection (Novachoice).
Cell Harvesting for Electrophysiology.
Cell Plating for Electrophysiology .
Freezing CHO Cells.
Plating Subclones for Ephys.
Plating Subclones for Fluorescent Screening.
Subcloning Cells.
Testing TREX Subclones Ephys.
[[Sack: Thawing CHOcells| Thawing CHO cells].
electrophysiology
General Lab
HPLC
BTAA Peptide Click HPLC 01/03/12.
Dylight Protocol 02/06/12.
Fmal Protocol.
HPLC Protocol.
Mass Spec Protocol for GxTx-13ACM.
Solutions
Ephys Solutions 08/15/12.
Example 20K External 11/08/11.
Reagent Stocks Template 11/21/11.
Solutions Template 11/22/11.
Molecular Biology
Bacteria Transformation (One Shot Top Ten Invitrogen).
Bacteria Transformation Invitrogen .
Growth of Bacterial Cultures .
Subcloning Efficiency DH5a Chemically Competent E. Coli .
Tentagel Azide Beads
Bead Cell Binding 05/15/12.
Beads Alykne Azide BTTAA 05/16/12.
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