Sack: Subcloning Cells: Difference between revisions
(New page: Besides reagents to trypsinize cells, you will need:<br> In hood:<br> 75 ml pre-warmed cell maintenance media (100ug/ml G418)<br> 3 TC treated 96-well plates<br> 2 Small tubes (1.5 m...) |
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Besides reagents to trypsinize cells, you will need:<br> | Besides reagents to trypsinize cells, you will need:<br> | ||
In hood:<br> | In hood:<br> |
Latest revision as of 16:12, 15 January 2013
Besides reagents to trypsinize cells, you will need: At microscope: Note: cells sink in media, so make sure to mix solution by pipetting up and down immediately before taking cells Rinse (DPBS), trypsinize, & resuspend cells in same volume media To count cells
Pipet >10 ul cells into small tube, remove from hood To dilute cells and plate into 96 well plates For 5 cells per 200 ul well, you will use a 25 cells/ml dilution: For 1 cells per 200 ul well, you will use a 5 cells/ml dilution: For 0.2 cells per 200 ul well, you will use a 1 cell/ml dilution: |