Difference between revisions of "Sack and Yarov-Yarovoy:Protocols"

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<br>
[[Sack: Cell Media with Selection Agents (Blasticidin, Zeocin, Geneticin)|Cell Media with Selection Agents (Blasticidin, Zeocin, Geneticin) 04/05/12]] [http://openwetware.org/images/2/27/Cell_Media_with_Gen_Selection_Agents_4-5-2012.docx  .]<br>
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==general lab==
[[Sack: Cell Media with Selection Agents (Blasticidin, Zeocin)|Cell Media with Selection Agents (Blasticidin, Zeocin) 11/15/11]] [http://openwetware.org/images/3/3a/Cell_Media_with_Selection_Agents_11-15-2011KE.docx  .]<br>
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[[Sack: Acidwash|Acidwash 03/22/11]][http://openwetware.org/images/9/9e/GLASSWARE_CLEANING_PROCEDURES.pdf   .]<br>
[[Sack: Cell Splitting Protocol|Cell Splitting Protocol 03/02/12]][http://openwetware.org/images/7/7f/Cell_Splitting_Protocol.doc  .]<br>
 
[[Sack: Cell Transfection (Lipofectamine LTX)|Cell Transfection (Lipofectamine LTX) 05/01/12]][http://openwetware.org/images/8/87/Cell_Transfection_%28Lipofectamine_LTX%29_5-01-2012_KE.doc]<br>
 
[[Sack: Cell Transfection (Lipofectamine)|Cell Transfection (Lipofectamine) 08/14/12]]<BR>
 
[[Sack: Cell Transfection (Novachoice)|Cell Transfection (Novachoice) 05/04/12]]<BR>
 
[[Sack: Cell Harvesting for Electrophysiology|Cell Harvesting for Electrophysiology 07/18/12]][http://openwetware.org/images/7/78/Cell_Harvesting_for_Electrophysiology_7-18-2012_KE.doc  .]<br>
 
[[Sack: Cell Plating for Electrophysiology|Cell Plating for Electrophysiology 10/21/11]] [http://openwetware.org/images/7/76/Cell_Plating_for_Electrophysiology_SackLab.doc   .]<br>
 
[[Sack: Freezing CHO cells|Freezing CHO Cells 04/05/12]]<BR>
 
[[Sack: Plating Subclones for Ephys|Plating Subclones for Ephys 09/29/11]]<BR>
 
[[Sack: Plating Subclones for Flourescent Screening|Plating Subclones for Flourescent Screening 04/05/12]]<BR>
 
[[Sack: Subcloning Cells| Subcloning Cells 02/23/11]]<BR>
 
[[Sack: Testing TREX Subclones Ephys| Testing TREX Subclones Ephys 04/06/11]]<BR>
 
[[Sack: Thawing CHOcells| Thawing CHO cells 07/09/12]]<BR>
 
 
  
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==cell culture==
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[[Sack: Cell Media with Selection Agents (Blasticidin, Zeocin, Geneticin)|Cell Media with Selection Agents (Blasticidin, Zeocin, Geneticin)]] [http://openwetware.org/images/8/84/Cell_Media_with_Gen_Selection_Agents_4-5-2012.pdf  .]<br>
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[[Sack: Cell Media with Selection Agents (Blasticidin, Zeocin)|Cell Media with Selection Agents (Blasticidin, Zeocin)]] [http://openwetware.org/images/e/e5/Cell_Media_with_Selection_Agents_11-15-2011KE.pdf  .]<br>
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[[Sack: Cell Splitting Protocol|Cell Splitting Protocol]][http://openwetware.org/images/a/ac/Cell_Splitting_Protocol.pdf  .]<br>
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[[Sack: Cell Transfection (Lipofectamine LTX)|Cell Transfection (Lipofectamine LTX)]][http://openwetware.org/images/e/e4/Cell_Transfection_%28Lipofectamine_LTX%29_5-01-2012_KE.pdf  .]<br>
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[[Sack: Cell Transfection (Lipofectamine)|Cell Transfection (Lipofectamine)]][http://openwetware.org/images/3/37/Cell_Transfection_%28Lipofectamine%29_8-14-2012_AS.pdf .]<BR>
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[[Sack: Cell Transfection (Novachoice)|Cell Transfection (Novachoice)]][http://openwetware.org/images/6/61/Cell_Transfection_%28Novachoice%29_5-04-2012_KE.pdf  .]<BR>
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[[Sack: Cell Harvesting for Electrophysiology|Cell Harvesting for Electrophysiology]][http://openwetware.org/images/4/48/Cell_Harvesting_for_Electrophysiology_7-18-2012_KE.pdf  .]<br>
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[[Sack: Cell Plating for Electrophysiology|Cell Plating for Electrophysiology]] [http://openwetware.org/images/1/1c/Cell_Plating_for_Electrophysiology_SackLab.pdf  .]<br>
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[[Sack: Freezing CHO cells|Freezing CHO Cells]][http://openwetware.org/images/5/52/Freezing_CHOcells_SackLab.pdf  .]<BR>
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[[Sack: Plating Subclones for Ephys|Plating Subclones for Ephys]][http://openwetware.org/images/d/dc/Plating_Subclones_for_Ephys_SackLab.pdf  .]<BR>
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[[Sack: Plating Subclones for Flourescent Screening|Plating Subclones for Fluorescent Screening]][http://openwetware.org/images/2/21/Plating_subclones_for_fluorescent_screening_SackLab.pdf  .]<BR>
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[[Sack: Subcloning Cells| Subcloning Cells]][http://openwetware.org/images/4/4a/Subcloning_Cells_SackLab_2-23-2011_KE.pdf  .]<BR>
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[[Sack: Testing TREX Subclones Ephys| Testing TREX Subclones Ephys]][http://openwetware.org/images/9/90/Testing_TREX_Subclones_Ephys.pdf  .]<BR>
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[[Sack: Thawing CHOcells| Thawing CHO cells]][http://openwetware.org/images/0/01/Thawing_CHOcells_SackLab_7-9-2012.pdf  .]<BR>
  
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==electrophys==
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[[Sack: Agar Bridge Formation| Agar Bridge Formation]][http://openwetware.org/images/a/ac/Agar_Bridges.pdf  .]<br>
  
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==molecular biology==
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[[Sack: Bacteria Transformation (One Shot Top Ten Invitrogen)| Bacteria Transformation (One Shot Top Ten Invitrogen)]][http://openwetware.org/images/8/8e/Bacteria_Transformation_%28One_Shot_Top10_Invitrogen%29.pdf  .]<br>
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Bacteria Transformation Invitrogen  [http://openwetware.org/images/0/00/Bacteria_Transformation_Invitrogen.pdf  .]<br>
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Growth of Bacterial Cultures [http://openwetware.org/images/3/39/QIAGEN_-_Growth_of_bacterial_cultures.pdf  .]<br>
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Subcloning Efficiency DH5a Chemically Competent E. Coli [http://openwetware.org/images/d/df/Subcloning_Efficiency_DH5a_Chemically_competent_E._Coli.pdf  .]<br>
  
 
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==solutions==
*Return to [[Sack]] Homepage
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Ephys Solutions 08/15/12[http://openwetware.org/images/3/3a/EPhys_Solutions_8-15-2012KE.xlsx  .]<br>
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Example 20K External 11/08/11[http://openwetware.org/images/8/8b/Example_20K_external_110811jon.xlsx  .]<br>
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Reagent Stocks Template 11/21/11[http://openwetware.org/images/0/00/Reagent_stocks_template_112111jon3.xls  .]<br>
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Solutions Template 11/22/11[http://openwetware.org/images/1/1b/Solutions_made.xls  .]<br>

Revision as of 11:27, 25 October 2012