Difference between revisions of "SYBR Gold"

From OpenWetWare
Jump to: navigation, search
(References)
 
Line 17: Line 17:
  
 
==References==
 
==References==
#[[Molecular Cloning]]
+
#[[doi:10.1101/pdb.prot4022|Molecular Cloning]]
 
#[http://probes.invitrogen.com/media/publications/120.pdf Technical information from Invitrogen]
 
#[http://probes.invitrogen.com/media/publications/120.pdf Technical information from Invitrogen]
 
#[http://probes.invitrogen.com/media/pis/mp11494.pdf SYBR Gold Nucleic Acid Gel Stain product info from Invitrogen]
 
#[http://probes.invitrogen.com/media/pis/mp11494.pdf SYBR Gold Nucleic Acid Gel Stain product info from Invitrogen]
 
#[http://probes.invitrogen.com/media/pis/td004.pdf General SYBR stain tips and tricks from Invitrogen]
 
#[http://probes.invitrogen.com/media/pis/td004.pdf General SYBR stain tips and tricks from Invitrogen]

Latest revision as of 14:12, 5 December 2006

Recommended by Molecular Cloning as the best of the SYBR dyes from Molecular Probes.

General information

  • ten-fold more sensitive than ethidium bromide.
  • greater dynamic range.
  • penetrates gels quickly.
  • Stains both DNA and RNA in conventional neutral polyacrylamide and agarose gels and in denaturing gels using urea, glyoxal or formaldehyde.
  • stain gels post-electrophoresis because SYBR Gold likely binds the charge phosphate backbone of nucleic acids altering the electrophoretic mobility significantly. (DNA bands can come out curved).
  • low background so no destaining is necessary.
  • can be excited at by standard transillumination at 300nm.
  • stained nucleic acids can be transferred to membranes for Northerns or Southerns.
  • doesn't inhibit most enzymatic reactions except PCR. PCR is sensitive to high dye concentrations. Address this issues by adjusting Mg2+ concentration or remove stain via ethanol precipitation.
  • very expensive
  • supplied at 10,000X concentration in anhydrous DMSO.
  • operates most efficiently between pH 7-8.5
  • stock solution is stable 6 months to 1 year at <= 20°C

References

  1. Molecular Cloning
  2. Technical information from Invitrogen
  3. SYBR Gold Nucleic Acid Gel Stain product info from Invitrogen
  4. General SYBR stain tips and tricks from Invitrogen