Difference between revisions of "SYBR Gold"

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==General information==
 
==General information==
*Ten-fold more sensitive than ethidium bromide.
+
*ten-fold more sensitive than ethidium bromide.
*Greater dynamic range.
+
*greater dynamic range.
*Penetrates gels quickly.
+
*penetrates gels quickly.
 
*Stains both DNA and RNA in conventional neutral polyacrylamide and agarose gels and in denaturing gels using urea, glyoxal or formaldehyde.
 
*Stains both DNA and RNA in conventional neutral polyacrylamide and agarose gels and in denaturing gels using urea, glyoxal or formaldehyde.
*Stain gels post-electrophoresis because SYBR Gold likely binds the charge phosphate backbone of nucleic acids altering the electrophoretic mobility significantly.  (DNA bands can come out curved).
+
*stain gels post-electrophoresis because SYBR Gold likely binds the charge phosphate backbone of nucleic acids altering the electrophoretic mobility significantly.  (DNA bands can come out curved).
*Low background so no destaining is necessary.
+
*low background so no destaining is necessary.
*Can be excited at by standard transillumination at 300nm.
+
*can be excited at by standard transillumination at 300nm.
*Stained nucleic acids can be transferred to membranes for Northerns or Southerns.
+
*stained nucleic acids can be transferred to membranes for Northerns or Southerns.
*Doesn't inhibit most enzymatic reactions except PCR.  PCR is sensitive to high dye concentrations.  Address this issues by adjusting Mg>sup>2+</sup> concentration or remove stain via ethanol precipitation.
+
*doesn't inhibit most enzymatic reactions except PCR.  PCR is sensitive to high dye concentrations.  Address this issues by adjusting Mg<sup>2+</sup> concentration or remove stain via ethanol precipitation.
*Very expensive
+
*very expensive
*Supplied at 10,000X concentration in anhydrous DMSO.
+
*supplied at 10,000X concentration in anhydrous DMSO.
 +
*operates most efficiently between pH 7-8.5
 +
*stock solution is stable 6 months to 1 year at <= 20&deg;C
  
 
==References==
 
==References==
#[[Molecular Cloning]]
+
#[[doi:10.1101/pdb.prot4022|Molecular Cloning]]
 +
#[http://probes.invitrogen.com/media/publications/120.pdf Technical information from Invitrogen]
 +
#[http://probes.invitrogen.com/media/pis/mp11494.pdf SYBR Gold Nucleic Acid Gel Stain product info from Invitrogen]
 +
#[http://probes.invitrogen.com/media/pis/td004.pdf General SYBR stain tips and tricks from Invitrogen]

Latest revision as of 14:12, 5 December 2006

Recommended by Molecular Cloning as the best of the SYBR dyes from Molecular Probes.

General information

  • ten-fold more sensitive than ethidium bromide.
  • greater dynamic range.
  • penetrates gels quickly.
  • Stains both DNA and RNA in conventional neutral polyacrylamide and agarose gels and in denaturing gels using urea, glyoxal or formaldehyde.
  • stain gels post-electrophoresis because SYBR Gold likely binds the charge phosphate backbone of nucleic acids altering the electrophoretic mobility significantly. (DNA bands can come out curved).
  • low background so no destaining is necessary.
  • can be excited at by standard transillumination at 300nm.
  • stained nucleic acids can be transferred to membranes for Northerns or Southerns.
  • doesn't inhibit most enzymatic reactions except PCR. PCR is sensitive to high dye concentrations. Address this issues by adjusting Mg2+ concentration or remove stain via ethanol precipitation.
  • very expensive
  • supplied at 10,000X concentration in anhydrous DMSO.
  • operates most efficiently between pH 7-8.5
  • stock solution is stable 6 months to 1 year at <= 20°C

References

  1. Molecular Cloning
  2. Technical information from Invitrogen
  3. SYBR Gold Nucleic Acid Gel Stain product info from Invitrogen
  4. General SYBR stain tips and tricks from Invitrogen