From OpenWetWare
Revision as of 05:39, 15 June 2009 by Jakob Suckale (talk | contribs)

SSC buffer is named after its ingredients saline (sodium chloride) and sodium citrate. It is used as a buffer for blotting, hybridisation, and stringency washes in Northern and Southern blots as well as DNA microarray.

1x SSC buffer

  • 150mM NaCl
  • 15mM sodium citrate

20x SSC

  • 800ml dH2O
  • 175.3g NaCl (3M)
  • 88.2g trisodium citrate (NaCit; 300mM; e.g. S4641)
  • adjust the pH to 7.0 with a few drops of 1M HCl
  • adjust the volume to 1L with dH2O
  • sterilize by autoclaving

using stock solutions

  • 3M NaCl
  • 300 mM sodium citrate, pH 7.0

See also