Difference between revisions of "SSC"

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(20x recipe)
(20x SSC)
 
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SSC buffer contains sodium chloride and sodium citrate.
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'''SSC buffer''' is named after its ingredients '''S'''aline (sodium chloride) and '''S'''odium '''C'''itrate. It is used as a buffer for blotting, hybridisation, and stringency washes in [[Northern]] and [[Southern blot]]s as well as DNA microarray.
  
 
== 1x SSC buffer ==
 
== 1x SSC buffer ==
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== 20x SSC ==
 
== 20x SSC ==
* 800ml dH2O
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* 800ml dH2O (RNase free if required)
 
* 175.3g [http://en.wikipedia.org/wiki/Nacl NaCl] (3M)
 
* 175.3g [http://en.wikipedia.org/wiki/Nacl NaCl] (3M)
 
* 88.2g [http://en.wikipedia.org/wiki/Trisodium_citrate trisodium citrate] (NaCit; 300mM; e.g. [http://www.sigmaaldrich.com/catalog/ProductDetail.do?N4=S4641|SIAL&N5=SEARCH_CONCAT_PNO|BRAND_KEY&F=SPEC S4641])
 
* 88.2g [http://en.wikipedia.org/wiki/Trisodium_citrate trisodium citrate] (NaCit; 300mM; e.g. [http://www.sigmaaldrich.com/catalog/ProductDetail.do?N4=S4641|SIAL&N5=SEARCH_CONCAT_PNO|BRAND_KEY&F=SPEC S4641])
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* sterilize by autoclaving
 
* sterilize by autoclaving
  
=== using stock solution ===
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=== using stock solutions ===
 
* 3M NaCl
 
* 3M NaCl
* 300 mM sodium citrate, pH 7.0  
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* 300 mM sodium citrate, pH 7.0
  
[[Category:Material]] and [[Category:Buffers]]
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== protocols using SSC ==
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* [[Dahlquist:DNA Microarray Protocol]]
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* [[Endy:Northern Blot, 32P End-Labeled Probes]]
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* [[20.109(S07): cDNA synthesis and microarray]]
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* [[20.109(F08): Mod 2 Day 7 cDNA synthesis and microarray]]
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== See also ==
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* [http://en.wikipedia.org/wiki/SSC_buffer Wikipedia: SSC buffer]
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[[Category:Material]]
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[[Category:Buffers]]

Latest revision as of 04:43, 15 June 2009

SSC buffer is named after its ingredients Saline (sodium chloride) and Sodium Citrate. It is used as a buffer for blotting, hybridisation, and stringency washes in Northern and Southern blots as well as DNA microarray.

1x SSC buffer

  • 150mM NaCl
  • 15mM sodium citrate

20x SSC

  • 800ml dH2O (RNase free if required)
  • 175.3g NaCl (3M)
  • 88.2g trisodium citrate (NaCit; 300mM; e.g. S4641)
  • adjust the pH to 7.0 with a few drops of 1M HCl
  • adjust the volume to 1L with dH2O
  • sterilize by autoclaving

using stock solutions

  • 3M NaCl
  • 300 mM sodium citrate, pH 7.0

protocols using SSC

See also