Difference between revisions of "SR"
(My favourite medium :-))
Latest revision as of 06:15, 22 July 2010
Super Rich Medium (aka SR Medium). This medium is optimized for high-yield protein expression in Escherichia coli.
- 20 g tryptone
- 20 g yeast extract
- 10 g beef extract
- 11 g glycerol
- 900 mL H2O
- 2.32 g KH2PO4
- 16.4 g K2HPO4*H2O
- 100 mL H2O
- Dissolve ingredients of both solutions.
- Pour into flasks or bottles (Solution A in 2 L flask or greater, Solution B in 250 mL flask or greater).
- Autoclave (liquid cycle)
Feil, I. (1992) PH.D. Thesis, Technische Universtiaet Carolo-Wilhelmina zu Braunschweig, Braunschweig, Germany