Difference between revisions of "SOC"

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#Follow directions to make 1 liter of [[SOB]] media
 
#Follow directions to make 1 liter of [[SOB]] media
 
#After cooling medium to less than 50°C, add 20 ml filter sterilized 20% glucose solution
 
#After cooling medium to less than 50°C, add 20 ml filter sterilized 20% glucose solution
 +
  
 
*2M glucose stock: dissolve 18 g glucose into 50 ml (final volume) ddH2O and filter-sterilize into sterile 50 ml tube
 
*2M glucose stock: dissolve 18 g glucose into 50 ml (final volume) ddH2O and filter-sterilize into sterile 50 ml tube
 
 
{| border="1" cellpadding="5" cellspacing="0" align="left"  
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{| border="1" cellpadding="5" cellspacing="0"
 
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! Reagent
 
! Reagent
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| 1 ml
 
| 1 ml
 
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*aliquote in 2 ml tubes
 
*aliquote in 2 ml tubes

Revision as of 20:08, 29 December 2011

Summary

SOC Medium. Used in Bacterial Transformation.

Ingredients

  • SOB
  • 20 mM glucose

Protocol

  1. Follow directions to make 1 liter of SOB media
  2. After cooling medium to less than 50°C, add 20 ml filter sterilized 20% glucose solution


  • 2M glucose stock: dissolve 18 g glucose into 50 ml (final volume) ddH2O and filter-sterilize into sterile 50 ml tube
Reagent for 1 L 500 mL 100 mL
2 M glucose 10 ml 5 ml 1 ml
  • aliquote in 2 ml tubes
  • store at -20°C

Source

Adapted From:

F. Ausubel et al., Short Protocols in Molecular Biology (John Wiley & Sons, ed. 4, 1999) pg. A1-36

Trivia

The "C" in SOC supposedly stands for catabolite repression due to the added glucose.