SIL072006-Construction of pSB1A2-J23016

From OpenWetWare
Revision as of 10:22, 24 July 2006 by Pineapplequeen (talk | contribs)
(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)
Jump to: navigation, search

F plasmid with trbC knockout
PCR JL12/JL13 on pKD3 (~1000bp, dPN1, Neb2)
Electroporate into pKD46xpOX38
Product is pSB1A2-J23016

JL12 Forward oligo to knockout trbC-f:
JL13 Reverse oligo to knockout trbC-f: