SIL072006-Construction of pSB1A2-J23016

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F plasmid with trbC knockout
PCR JL12/JL13 on pKD3 (~1000bp, dPN1, Neb2)
Electroporate into pKD46xpOX38
Product is pSB1A2-J23016


JL12 Forward oligo to knockout trbC-f:
atgaagctgagtatgaaatctctggcagcactgctgatgatgctggtgCaggctggagctgcttcgaag
JL13 Reverse oligo to knockout trbC-f:
tcatttcccggaatctcctttccctgccagtaaatcatgagccactgggaaGtagccatggtccatat