SIL071906-Construction of pSB1A2-J23014

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[trbCf]
PCR JL14/JL15 on Olambda (679bp, EcoRI/SpeI, Neb2)
Paste into pSB1A2-I13521 (EcoRI/SpeI, Neb2, Large 2062)
Product is pSB1A2-J23014


JL14 Forward EcoRI oligo to bioBrick trbC:
CCTAGGAATTCGCGGCCGCTTCTAGatgaagctgagtatgaaatc
JL15 Reverse SpeI oligo to bioBrick trbC:
gacttACTAGTcgtttcatttcccggaatctc

JL16 Forward trbCf 20mer
gctggcgttaccagatggtg
JL17 Reverse trbCf 20mer
gccctgaatactgctgcttcc