Difference between revisions of "SEED/2010/Day 7"

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(New page: == Morning == * Discuss sequencing technologies * Review labs so far == Afternoon == * pick 1 blue/green colony per plate for growth and templiphi in strips ** pick 4 colonies per group *...)
 
(No difference)

Latest revision as of 09:39, 2 February 2010

Morning

  • Discuss sequencing technologies
  • Review labs so far

Afternoon

  • pick 1 blue/green colony per plate for growth and templiphi in strips
    • pick 4 colonies per group
    • 2.5ul sample buffer per tube
    • pick colony and dip in tube
    • 3m@95C to break open cells
    • Add 2.5ul reaction buffer + 0.1ul enzyme mix
  • incubate templiphi at 30C overnight
  • Class project mini-presentations/discussions

Instructor Post-prep

  • heat kill templiphi
  • transfer 2ul of templiphi into sequencing mixes with VF2
  • Send out for sequencing