Difference between revisions of "SDS-PAGE"

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(Solutions for various polyacrylamide gels (SDS-PAGE))
(Solutions for 10 ml (MINIgel) of various polyacrylamide gels (SDS-PAGE))
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===Solutions for 10 ml (MINIgel) of various polyacrylamide gels (SDS-PAGE)===
 
===Solutions for 10 ml (MINIgel) of various polyacrylamide gels (SDS-PAGE)===
 
  Chemical      8%    10%    12%
 
  Chemical      8%    10%    12%
 +
----
 
  Water        4.74  4.0    3.3  ml
 
  Water        4.74  4.0    3.3  ml
 
  30% AA(*)    2.66  3.3    4.0  ml
 
  30% AA(*)    2.66  3.3    4.0  ml
 
  LOWER buffer  2.5    2.5    2.5  ml
 
  LOWER buffer  2.5    2.5    2.5  ml

Revision as of 02:08, 15 May 2007

Solutions for 10 ml (MINIgel) of various polyacrylamide gels (SDS-PAGE)

Chemical      8%     10%     12%

Water         4.74   4.0     3.3  ml
30% AA(*)     2.66   3.3     4.0  ml
LOWER buffer  2.5    2.5     2.5  ml