Difference between revisions of "SBB13 gels"

From OpenWetWare
Jump to: navigation, search
(JCAnderson 11:26, 13 March 2013 (EDT))
Line 2: Line 2:
 
[[Image:2013_03_13-JCA-gel1.jpg | 200px ]]<br>
 
[[Image:2013_03_13-JCA-gel1.jpg | 200px ]]<br>
  
This gel is of the PCA2 products for the various synthons.  The first lane is the His-tag part's IPCR product (currently unnamed), then molecular weight standards, then the rest are your PCA2's (I couldn't read the labels).  All but 3 look fine.  Lanes 2 and 6 look recoverable with a second PCR.  The lane 1 reaction needs to be started over from scratch.  Lane 5 just needs a careful gel purification.
+
This gel is of the PCA2 products for the various synthons.  The first lane is the His-tag part's IPCR product (currently unnamed), then molecular weight standards, then the rest are your PCA2's (I couldn't read the labels).  All but three look fine.  Lanes 2 and 6 look recoverable with a second PCR.  The lane 1 reaction needs to be started over from scratch.  Lane 5 just needs a careful gel purification.

Revision as of 07:31, 13 March 2013

JCAnderson 11:26, 13 March 2013 (EDT)

2013 03 13-JCA-gel1.jpg

This gel is of the PCA2 products for the various synthons. The first lane is the His-tag part's IPCR product (currently unnamed), then molecular weight standards, then the rest are your PCA2's (I couldn't read the labels). All but three look fine. Lanes 2 and 6 look recoverable with a second PCR. The lane 1 reaction needs to be started over from scratch. Lane 5 just needs a careful gel purification.