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== [[User:Susan C. Roberts|Susan C. Roberts]] ==
== [[User:Susan C. Roberts|Susan C. Roberts]] ==
[[Image:SusanCRoberts.png|right|100px]]Sue is an associate professor in the [http://che.umass.edu/ Chemical Engineering Department] at [http://www.umass.edu/ The University of Massachusetts Amherst]. She also holds a position as the director of the UMass Amherst Institute for Cellular Engineering ([http://www.umass.edu/ice/ ICE]). She obtained her B.S. in Chemical Engineering from Worcester Polytechnic Institute in 1992 and Ph.D. in Chemical Engineering from Cornell University in 1998.<br>
[[Image:SusanCRoberts.png|right|100px]]Sue is an associate professor in the [http://che.umass.edu/ Chemical Engineering Department] at [http://www.umass.edu/ The University of Massachusetts Amherst]. She also holds a position as the director of the UMass Amherst Institute for Cellular Engineering ([http://www.umass.edu/ice/ ICE]). She obtained her B.S. in Chemical Engineering from Worcester Polytechnic Institute in 1992 and Ph.D. in Chemical Engineering from Cornell University in 1998.<br>
[http://che.umass.edu/faculty/susan-roberts Contact Information:]<br>
[http://che.umass.edu/faculty/susan-roberts Contact Information:]<br>
Email: sroberts@ecs.umass.edu
Email: sroberts@ecs.umass.edu<br>
 
[[Media:SCRoberts_CV.pdf | Sue's Current CV]]<br>
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== Current Graduate Students ==
== Current Graduate Students ==
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[[Image:MartinEKolewe.png|right|100px]]'''Martin Kolewe''' completed his Ph.D. in Chemical Engineering at UMass Amherst in 2011 and his B.S. in Chemical Engineering at Johns Hopkins University in 2004. After completing his undergraduate degree, he worked for 3 years as a process control engineer for Amgen in the start up of their BioNext manufacturing facility. He joined the [http://roberts.openwetware.org/ Roberts lab] in 2007 and studied heterogeneity in plant cell culture through both modeling and experimental techniques, collaborating with [http://che.umass.edu/faculty/michael-henson Mike Henson]. '''Marty is currently searching for a post-doctoral position in the Boston Area''' and spending time with his 1-year old daughter, Artemis and wife, Christina.<br>
Email: mkolewe@ecs.umass.edu<br>
[[Media:MartinEKolewe_CV.pdf | Marty's Current CV]]<br>
----
[[Image:RohanPatil.jpg|right|100px]]'''Rohan Patil''' completed his B.S. in Chemical engineering at University Institute of Chemical Technology, Mumbai, India. He joined [http://roberts.openwetware.org/ Roberts lab] in 2008. The general aim of Rohan’s research is to better understand the heterogeneity in plant cell cultures, with a focus on elucidating the relationship between cell populations and paclitaxel accumulation in culture. He is presently working on the application of molecular approaches to characterize and manipulate Taxus metabolism in distinct cell subpopulations.<br>
Email: patil@ecs.umass.edu <br>
[[Media:RohanPatil_CV.pdf | Rohan's Current CV]]<br>
<br>
<br>
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[[Image:WhitneyLStoppel.png|right|102px]][[User:Whitney L. Stoppel|'''Whitney Stoppel''']] completed her B.S. in Chemical Engineering with minors in Mathematics and Cell & Molecular Biology at Tulane University in 2008. Following an REU program in [http://www.umass.edu/ice/reu/index.html Cellular Engineering] at UMass in 2007, Whitney chose to attend graduate school at UMass, continuing to work in the [http://roberts.openwetware.org/ Roberts lab]. She joined the Roberts lab in the fall of 2008, and is working on enhancing the growth and functionality of mammalian cells in 3-D constructs by tailoring mechanical and physiological properties within the device through a collaboration with [http://che.umass.edu/faculty/surita-bhatia Surita Bhatia], [http://www.bio.umass.edu/mcb/faculty/Tremblay.html Kim Tremblay], and [http://www.pse.umass.edu/~acrosby/ Al Crosby]. <br>
[[Image:WhitneyLStoppel.png|right|102px]][[User:Whitney L. Stoppel|'''Whitney Stoppel''']] completed her B.S. in Chemical Engineering with minors in Mathematics and Cell & Molecular Biology at Tulane University in 2008. Following an REU program in [http://www.umass.edu/ice/reu/index.html Cellular Engineering] at UMass in 2007, Whitney chose to attend graduate school at UMass, continuing to work in the [http://roberts.openwetware.org/ Roberts lab]. She joined the Roberts lab in the fall of 2008, and is working on enhancing the growth and functionality of mammalian cells in 3-D constructs by tailoring mechanical and physiological properties within the device through a collaboration with [http://che.umass.edu/faculty/surita-bhatia Surita Bhatia], [http://www.bio.umass.edu/mcb/faculty/Tremblay.html Kim Tremblay], and [http://www.pse.umass.edu/~acrosby/ Al Crosby]. <br>
Email: wstoppel@ecs.umass.edu <br>
Email: wstoppel@ecs.umass.edu <br>
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[[Image:SarahAWilson.jpg|right|100px]][[User:Sarah A. Wilson|'''Sarah Wilson''']] completed her B.S. in Chemical Engineering at Rowan University in the spring of 2009. She decided to attend graduate school at UMass to conduct research in cellular engineering and joined the Roberts group in the fall of 2009. Sarah's focus is on characterizing novel genes involved in paclitaxel production with an overall goal to enhance the accumulation and production of paclitaxel in Taxus cell cultures.<br>
[[Image:SarahAWilson.png|right|100px]][[User:Sarah A. Wilson|'''Sarah Wilson''']] completed her B.S. in Chemical Engineering at Rowan University in the spring of 2009. She decided to attend graduate school at UMass to conduct research in cellular engineering and joined the Roberts group in the fall of 2009. Sarah's focus is on characterizing novel genes involved in paclitaxel production with an overall goal to enhance the accumulation and production of paclitaxel in ''Taxus'' cell cultures through agrobacterium-mediated transformation. She is completing this work in collaboration with [http://bti.cornell.edu/JoyceVanEck.php#page=ResearchSummary Joyce Van Eck] at the Boyce Thompson Institute for Plant Research in Ithaca, New York.<br>
<br>
<br>
Email: swilson@ecs.umass.edu <br>
Email: swilson@ecs.umass.edu <br>
<br>
<br>
<br>
<br>
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[[Image:LisaLeone.png|right|100px]][[User:Lisa M. Leone|'''Lisa Leone''']] completed her B.S. in Chemical Engineering at Drexel University in Philadelphia. During the course of her undergraduate education she participated in internship programs at ConocoPhillips, Dupont and Genencor, the last of which sparked an interest in biotechnology. She joined the Roberts group in the fall of 2010, and looks forward to optimizing Taxol production through metabolic engineering.<br>
[[Image:LisaLeone.png|right|100px]][[User:Lisa M. Leone|'''Lisa Leone''']] completed her B.S. in Chemical Engineering at Drexel University in Philadelphia. During the course of her undergraduate education she participated in internship programs at ConocoPhillips, Dupont and Genencor, the last of which sparked an interest in biotechnology. She joined the Roberts group in the fall of 2010 to optimize plant cell growth in bioreactors through a combination of mathematical modelling and experimental techniques in collaboration with [http://che.umass.edu/faculty/michael-henson Mike Henson].<br>
<br>
<br>
Email: lleone@ecs.umass.edu <br>
Email: lleone@ecs.umass.edu <br>
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*[http://che.umass.edu/faculty/michael-henson Mike Henson] in the [http://che.umass.edu/ Chemical Engineering Department at UMass Amherst]
*[http://che.umass.edu/faculty/michael-henson Mike Henson] in the [http://che.umass.edu/ Chemical Engineering Department at UMass Amherst]
*[http://www.biochem.umass.edu/normanly/ Jennifer Normanly] in the [http://www.biochem.umass.edu/ Biochemistry and Molecular Biology Department at UMass Amherst]
*[http://www.biochem.umass.edu/normanly/ Jennifer Normanly] in the [http://www.biochem.umass.edu/ Biochemistry and Molecular Biology Department at UMass Amherst]
*[http://www.bio.umass.edu/mcb/faculty/Tremblay.html Kimberly Tremblay] in the [http://www.umass.edu/vasci/ Veterinary and Animal Science Department at UMass Amherst]
*[http://bti.cornell.edu/JoyceVanEck.php#page=ResearchSummary Joyce Van Eck] at the [http://bti.cornell.edu/ Boyce Thompson Institute for Plant Research]
*[http://bti.cornell.edu/JoyceVanEck.php#page=ResearchSummary Joyce Van Eck] at the [http://bti.cornell.edu/ Boyce Thompson Institute for Plant Research]
*[http://www.bio.umass.edu/biology/about/directories/faculty/elsbeth-walker Elsbeth Walker] in the [http://www.bio.umass.edu/biology/ Biology Department at UMass Amherst]
*[http://www.bio.umass.edu/biology/about/directories/faculty/elsbeth-walker Elsbeth Walker] in the [http://www.bio.umass.edu/biology/ Biology Department at UMass Amherst]
----
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== Current Undergraduate Researchers ==
== Current Undergraduate Researchers ==
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[[Image:NickCadirov.jpg|right|100px]]'''Nick Cadirov''' will graduate with his B.S. in Chemical Engineering in May 2012 from the University of Massachusetts Amherst and is currently applying to graduate school for the fall 2012 semester. He has worked in the Roberts lab since the beginning of his junior year. As a senior, he is currently completing his honors thesis, working to optimize a protocol for the reculturing of ''Taxus'' protoplasts. In the spring of 2011, Nick presented his work at the annual UMass Undergraduate Research symposium.  
[[Image:KelseyMantoni.png|101px|right]]'''Kelsey Mantoni''' joined the Roberts Lab in the fall of 2011 as a sophomore honor chemical engineering student to begin work on her Capstone honors thesis. Kelsey will be working with Whitney in the field of biomaterials and tissue engineering.  
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<br>
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'''Michael Vilkhavoy''' joined the Roberts Lab in the fall of 2011 as a sophomore honor chemical engineering student to begin work on his Capstone honors thesis. Michael will be working with Sarah on plant cell culture transformation.
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[[Image:SarenaDHorava.png|100px|right]]'''Sarena Horava''' will graduate with his B.S. in Chemical Engineering in May 2012 from the University of Massachusetts Amherst and is currently applying to graduate school for the fall 2012 semester. She has worked in the field of biomaterials and tissue engineering in the Roberts lab since the beginning of her sophomore year. She has received numerous UMass Commonwealth Honors College fellowships and has presented a poster at the UMass Undergraduate Research Symposium in the spring of 2010 and 2011. This fall, she will present her work at the BMES annual meeting in Hartford, CT during the undergraduate poster session. Currently, she is completing her honors thesis through the exploration of smooth muscle cell extracellular matrix secretion in alginate hydrogels over time.
'''Alec Gramann''' joined the Roberts Lab in the fall of 2011 as a sophomore chemical engineering student to begin work on plant cell culture dynamics in stirred bioreactors with Lisa.
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[[Image:NicoleRaia.jpg|100px|right]] '''Nicole Raia''' will graduate with her B.S. in Chemical Engineering in May 2012 from the University of Massachusetts Amherst and is currently applying to graduate school and industry positions. Nicole has worked in the Roberts since the beginning of her junior year. As a senior, she is currently completing her honors thesis, working to develop a selection protocol for the Roberts lab's ''Taxus'' cell lines after an Agrobacterium-mediated transformation. As a result of her hard work, Nicole received a Commonwealth Honors College fellowship in the spring of 2011 and presented her results at the annual UMass Undergraduate Research symposium.  
'''William Tyler Frantz''' joined the Roberts Lab in the fall of 2011 as a sophomore chemical engineering student to begin work on plant cell culture dynamics in stirred bioreactors with Lisa.  
<br>
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'''Steven Bevacqua''' will join the Roberts Lab in the fall of 2012 as a sophomore chemical engineering student to begin work on plant cell culture with Sarah.
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== Former Postdoctoral Researchers ==
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*'''Ling Tao''' (2004-2005) ''“Optimizing fuel metabolism and delivery for diabetes treatment.”'' (co-advisor S. Bhatia) Current position: National Renewable Energy Laboratory, Golden, CO
*'''Nadia Boutaoui''' (2003-2006), ''“Differential mRNA expression analysis in Taxus cells in response to methyl jasmonate elicitation.”'' (co-advisor E. Walker) Current position: Brigham and Women’s Hospital, Boston, MA


== Former PhD Students ==
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[[Image:RobertsGroupWinter2009.png|right|400px|]]
== Former Graduate Students ==
*Martin Kolewe
*Vishal Gaurav
*Kham Vongpaseuth
*Kyong-Sik Chin
*Sarwat F. Khattak
*Michael C. Naill
*Camille Dubois
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*'''Rohan Patil''' [[Image:RohanPatil.png|right|101px]] (ChE, PhD), 2012, ''"Molecular and population level approaches to understand ''Taxus'' metabolism in cell suspension cultures."''Rohan is currently employed at Genzyme.
*'''Martin E. Kolewe''' [[Image:MartinEKolewe.png|right|101px]](ChE, PhD), 2011, ''“Plant cell heterogeneity, aggregation and population dynamics.”'' Marty is currently a post-doc under Dr. Lisa Freed and Dr. Robert Langer at Draper Laboratory.
Email: mkolewe@mit.edu<br>
*'''Vishal Gaurav''' (ChE, PhD), 2011, ''“Flow cytometry of cultured plant cells for characterization of culture heterogeneity and cell sorting applications.”'' Current position: BioGenex, MA.
*'''Kham Vongpaseuth''' (Plant Biology, PhD), 2011, ''“Taxus transformation and functional characterization of putative regulatory genes.”'' Current position: United States Department of Agriculture – Animal and Plant Health Inspection Service – Biotechnology Regulatory Services (USDA-APHIS-BRS), Riverdale, MD
*'''KyuongSik Chin''' (ChE, PhD), 2009, ''“Optimizing cell encapsulation devices through enhanced oxygen supply.”'' Current position: Samsung, Korea
*'''Sarwat Khattak''' (ChE, PhD), 2006, ''“In vitro applications in drug metabolism and cell encapsulation.”'' Current position: Bristol-Myers Squibb, Syracuse, NY
*'''Michael Naill''' (ChE, PhD), 2005, ''“The use of flow cytometry to study variability of paclitaxel accumulation in Taxus cell suspension cultures”'' Current position: Abbott Laboratories, Worcester, MA
*'''Camille Dubois''' (ChE, MS), 2005, ''“Profiling taxanes in plant cell suspension cultures.”'' Current Position: DianaPlantSciences, Inc, Portland, OR
*'''Jeanne Michon''' (ChE, MS), 2001, ''“Understanding taxane metabolism through the development of a cell-free system and studies on taxane degradation.”''
*'''Julian Esclassan''' (ChE, MS), 2001, ''“Paclitaxel stability in plant cell suspension cultures.”''
----
== Former Undergraduates with Honors Theses ==
== Former Undergraduates with Honors Theses ==
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*Jordan Atlas
*David Babson
*Nick Cadirov
*Meenal Datta
*Nikola Finneran
*Katie Geldhart
*Daniel Hines
*Sarena Horava
*Michael Lovett
*Julie Matthew
*Gretchen McAuliffe
*Jonathan Moreno
*Nicole Raia
*Eva Shah
*Amit Shavit
*Amit Shavit
*Nikola Finneran
*Michelle Spatara
*Meenal Datta
*Melissa St. Amand
*Melissa St. Amand
*Jordan Atlas
----
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==Former REU Students==
==Former REU Students==
'''Summer 2012'''
*Emily Hartzell, Institute for Cellular Engineering REU Student
*Julia Petrullo, Institute for Cellular Engineering REU Student<br>
'''Summer 2011'''
'''Summer 2011'''
*Nathan Bade, Institute for Cellular Engineering REU Student
*Nathan Bade, Institute for Cellular Engineering REU Student
*Kristin Garcia, Institute for Cellular Engineering REU Student<br>
*Kristen Garcia, Institute for Cellular Engineering REU Student<br>
'''Summer 2010'''
'''Summer 2010'''
*[http://www.umass.edu/ice/images/reu_highlights/Meghan%20Combs_res_high%20ppt.pdf Meghan Combs], Institute for Cellular Engineering REU Student
*[http://www.umass.edu/ice/images/reu_highlights/Meghan%20Combs_res_high%20ppt.pdf Meghan Combs], Institute for Cellular Engineering REU Student
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'''Summer 2008'''
'''Summer 2008'''
*[http://www.umass.edu/ice/images/reu_highlights/koridunn_res_high.pdf Kori Dunn], Institute for Cellular Engineering REU Student<br>
*[http://www.umass.edu/ice/images/reu_highlights/koridunn_res_high.pdf Kori Dunn], Institute for Cellular Engineering REU Student<br>
'''Summer 2007'''
'''Before 2008'''
*Whitney Stoppel, Institute for Cellular Engineering REU student
*Whitney Stoppel
*Sally Wang
*Gaby Mas
*Robert Harmon
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==Open Positions==
The Roberts lab is always willing to take REU students funded through programs on the UMass campus. Please see the [http://www.umass.edu/ice/reu/index.html Institute for Cellular Engineering REU program] and the [http://www-new.ecs.umass.edu/reu UMass College of Engineering REU program] for opportunities.
'''Post-doctoral positions'''<br>
The Roberts Lab does not have any post-doctoral positions open at this time.<br>
 
'''Graduate Students'''<br>
The Roberts Lab may be interested in a qualified chemical engineering graduate student in the fall of 2011. Check back soon for more information. <br>
 
'''Undergraduate Students'''<br>
The Roberts lab is currently filling its positions for the 2011-2012 academic year, but may have openings in the fall of 2012 for juniors wishing to complete an honors thesis the following year. Please check back in the spring for more information and an application. <br>
 
'''Summer REU Students'''<br>
The Roberts lab is always willing to take REU students funded through programs on the UMass campus. Please check out the [http://www.umass.edu/ice/reu/index.html Institute for Cellular Engineering REU program] and the [http://www-new.ecs.umass.edu/reu UMass College of Engineering REU program] for opportunities.
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Revision as of 09:21, 27 September 2012


Home        People        Publications        Research        Presentations        Courses        News        Internal        ICE   


Susan C. Roberts

Sue is an associate professor in the Chemical Engineering Department at The University of Massachusetts Amherst. She also holds a position as the director of the UMass Amherst Institute for Cellular Engineering (ICE). She obtained her B.S. in Chemical Engineering from Worcester Polytechnic Institute in 1992 and Ph.D. in Chemical Engineering from Cornell University in 1998.

Contact Information:
Email: sroberts@ecs.umass.edu
Sue's Current CV


Current Graduate Students


Whitney Stoppel completed her B.S. in Chemical Engineering with minors in Mathematics and Cell & Molecular Biology at Tulane University in 2008. Following an REU program in Cellular Engineering at UMass in 2007, Whitney chose to attend graduate school at UMass, continuing to work in the Roberts lab. She joined the Roberts lab in the fall of 2008, and is working on enhancing the growth and functionality of mammalian cells in 3-D constructs by tailoring mechanical and physiological properties within the device through a collaboration with Surita Bhatia, Kim Tremblay, and Al Crosby.

Email: wstoppel@ecs.umass.edu


Sarah Wilson completed her B.S. in Chemical Engineering at Rowan University in the spring of 2009. She decided to attend graduate school at UMass to conduct research in cellular engineering and joined the Roberts group in the fall of 2009. Sarah's focus is on characterizing novel genes involved in paclitaxel production with an overall goal to enhance the accumulation and production of paclitaxel in Taxus cell cultures through agrobacterium-mediated transformation. She is completing this work in collaboration with Joyce Van Eck at the Boyce Thompson Institute for Plant Research in Ithaca, New York.


Email: swilson@ecs.umass.edu


Lisa Leone completed her B.S. in Chemical Engineering at Drexel University in Philadelphia. During the course of her undergraduate education she participated in internship programs at ConocoPhillips, Dupont and Genencor, the last of which sparked an interest in biotechnology. She joined the Roberts group in the fall of 2010 to optimize plant cell growth in bioreactors through a combination of mathematical modelling and experimental techniques in collaboration with Mike Henson.


Email: lleone@ecs.umass.edu


Current Collaborators



Current Undergraduate Researchers


Kelsey Mantoni joined the Roberts Lab in the fall of 2011 as a sophomore honor chemical engineering student to begin work on her Capstone honors thesis. Kelsey will be working with Whitney in the field of biomaterials and tissue engineering.





Michael Vilkhavoy joined the Roberts Lab in the fall of 2011 as a sophomore honor chemical engineering student to begin work on his Capstone honors thesis. Michael will be working with Sarah on plant cell culture transformation.


Alec Gramann joined the Roberts Lab in the fall of 2011 as a sophomore chemical engineering student to begin work on plant cell culture dynamics in stirred bioreactors with Lisa.


William Tyler Frantz joined the Roberts Lab in the fall of 2011 as a sophomore chemical engineering student to begin work on plant cell culture dynamics in stirred bioreactors with Lisa.


Steven Bevacqua will join the Roberts Lab in the fall of 2012 as a sophomore chemical engineering student to begin work on plant cell culture with Sarah.


Former Postdoctoral Researchers


  • Ling Tao (2004-2005) “Optimizing fuel metabolism and delivery for diabetes treatment.” (co-advisor S. Bhatia) Current position: National Renewable Energy Laboratory, Golden, CO
  • Nadia Boutaoui (2003-2006), “Differential mRNA expression analysis in Taxus cells in response to methyl jasmonate elicitation.” (co-advisor E. Walker) Current position: Brigham and Women’s Hospital, Boston, MA

Former Graduate Students


  • Rohan Patil
    (ChE, PhD), 2012, "Molecular and population level approaches to understand Taxus metabolism in cell suspension cultures."Rohan is currently employed at Genzyme.
  • Martin E. Kolewe
    (ChE, PhD), 2011, “Plant cell heterogeneity, aggregation and population dynamics.” Marty is currently a post-doc under Dr. Lisa Freed and Dr. Robert Langer at Draper Laboratory.

Email: mkolewe@mit.edu

  • Vishal Gaurav (ChE, PhD), 2011, “Flow cytometry of cultured plant cells for characterization of culture heterogeneity and cell sorting applications.” Current position: BioGenex, MA.
  • Kham Vongpaseuth (Plant Biology, PhD), 2011, “Taxus transformation and functional characterization of putative regulatory genes.” Current position: United States Department of Agriculture – Animal and Plant Health Inspection Service – Biotechnology Regulatory Services (USDA-APHIS-BRS), Riverdale, MD
  • KyuongSik Chin (ChE, PhD), 2009, “Optimizing cell encapsulation devices through enhanced oxygen supply.” Current position: Samsung, Korea
  • Sarwat Khattak (ChE, PhD), 2006, “In vitro applications in drug metabolism and cell encapsulation.” Current position: Bristol-Myers Squibb, Syracuse, NY
  • Michael Naill (ChE, PhD), 2005, “The use of flow cytometry to study variability of paclitaxel accumulation in Taxus cell suspension cultures” Current position: Abbott Laboratories, Worcester, MA
  • Camille Dubois (ChE, MS), 2005, “Profiling taxanes in plant cell suspension cultures.” Current Position: DianaPlantSciences, Inc, Portland, OR
  • Jeanne Michon (ChE, MS), 2001, “Understanding taxane metabolism through the development of a cell-free system and studies on taxane degradation.”
  • Julian Esclassan (ChE, MS), 2001, “Paclitaxel stability in plant cell suspension cultures.”

Former Undergraduates with Honors Theses


  • Jordan Atlas
  • David Babson
  • Nick Cadirov
  • Meenal Datta
  • Nikola Finneran
  • Katie Geldhart
  • Daniel Hines
  • Sarena Horava
  • Michael Lovett
  • Julie Matthew
  • Gretchen McAuliffe
  • Jonathan Moreno
  • Nicole Raia
  • Eva Shah
  • Amit Shavit
  • Michelle Spatara
  • Melissa St. Amand

Former REU Students

Summer 2012

  • Emily Hartzell, Institute for Cellular Engineering REU Student
  • Julia Petrullo, Institute for Cellular Engineering REU Student

Summer 2011

  • Nathan Bade, Institute for Cellular Engineering REU Student
  • Kristen Garcia, Institute for Cellular Engineering REU Student

Summer 2010

  • Meghan Combs, Institute for Cellular Engineering REU Student
  • Vanessa Mukania, UMass College of Engineering REU Student

Summer 2009

Summer 2008

  • Kori Dunn, Institute for Cellular Engineering REU Student

Before 2008

  • Whitney Stoppel
  • Sally Wang
  • Gaby Mas
  • Robert Harmon

The Roberts lab is always willing to take REU students funded through programs on the UMass campus. Please see the Institute for Cellular Engineering REU program and the UMass College of Engineering REU program for opportunities.


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