Difference between revisions of "Rifampicin"
(Suggestion for how to handle common references. Change back if desired.)
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A Short Course in Bacterial Genetics
Revision as of 13:58, 23 May 2005
Mode of Action
Strongly inhibits prokaryotic RNA polymerase and mammalian RNA polymerase to a lesser degree.
Mechanism of Resistance
Cells can spontaneously mutate to achieve resistance to Rifampicin.
- 50 mg/mL stock solution in methanol.
- Vortex immediately to prevent rifampicin from sticking to bottom of tube.
- Add ~5 drops 10 N NaOH per mL to facilitate dissolving.
- Add 2 mL per liter of culture to achieve a working concentration of 100 μg/mL.
Rifampicin is light-sensitive so store stock solution, plates and media in the dark and wrap in foil.