Difference between revisions of "Rifampicin"

From OpenWetWare
Jump to: navigation, search
(Suggestion for how to handle common references. Change back if desired.)
Line 18: Line 18:
J. H. Miller. ''A Short Course in Bacterial Genetics: A Laboratory Manual and Handbook for Escherichia coli and Related Bacteria.'' Cold Spring Harbor Laboratory Press, 1992.
*[[A Short Course in Bacterial Genetics]]
*[[Molecular Cloning]]
J. Sambrook and D. W. Russell. ''Molecular Cloning: A Laboratory Manual.'' Cold Spring Harbor Laboratory Press, 3rd edition, 2001.

Revision as of 13:58, 23 May 2005

Mode of Action

Strongly inhibits prokaryotic RNA polymerase and mammalian RNA polymerase to a lesser degree.

Mechanism of Resistance

Cells can spontaneously mutate to achieve resistance to Rifampicin.

Usage Notes

  1. 50 mg/mL stock solution in methanol.
  2. Vortex immediately to prevent rifampicin from sticking to bottom of tube.
  3. Add ~5 drops 10 N NaOH per mL to facilitate dissolving.
  4. Add 2 mL per liter of culture to achieve a working concentration of 100 μg/mL.

Rifampicin is light-sensitive so store stock solution, plates and media in the dark and wrap in foil.