Difference between revisions of "Richard Lab:protocols"

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(Molecular/Synthetic Biology)
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==Dionex IC==
==Dionex IC==
*[[Richard Lab:IC standards]]
*[[Richard Lab:IC standards|IC Standard Preparation]]
==Dionex ASE==
==Dionex ASE==
*Put protocol here
*Put protocol here
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==Fiber Analysis==
==Fiber Analysis==
::'''[[Richard_Lab:_NDF|NDF (Neutral Detergent Fiber)]]'''

Revision as of 10:36, 3 February 2010

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Dionex IC

Dionex ASE

  • Put protocol here

Anaerobic Chamber

  • Put protocol here

Data Logger

To Reconfigure data into a compatible format


Molecular/Synthetic Biology

Plasmid Extraction
DNA Quantification
DNA Concentration
DNA Synthesis from Oligos
Bio-Brick Digest
Partial Restriction Digest
Agarose Gel Electrophoresis
Polyacrylamide Gel Electrophoresis
Gel Extraction of DNA
Electro-transformation of E. coli
Electro-transformation of Lactobacillus
Making Electro-competent Cells
Promoter Probing

Fiber Analysis

NDF (Neutral Detergent Fiber)
TMAH Lignin Analysis
BOD, moisture and VS content

Manure Storage and Emissions Analysis

Measuring and Analyzing Gas-Flux from a Surface

  • gaseous emission data is collected using a static flux chamber and a photoacoustic sensor.
  • an Excel program is then used to extract relevant data (from the continuous data collected by the photoacoustic sensor every minute). The same program also calculates the flux of selected gases from the tested surface, using Pedersen's equation (2001).
  • selected data are copied and pasted as values into a compiled data set
  • a regression analysis is performed to evaluate the effect of time, biofilter treatment, and other input variables on the flux level of each analyzed gas: ammonia, methane, carbon dioxide, nitrous oxide and water vapor. The Sagata Regression Pro software (ltd) is used: go to regression > pick inputs > pick outputs > chose data types (categorical or continuous) > chose regression model (to evaluate interactions create input variables made of factors evaluate for interaction amongst them, multiplied by each other) > go to output, get response vs. factors for preliminary graphs > choose indicative title for the output sheet > click ok.
  • the statistical analysis output is interpreted: the first Anova table shows possible treatment effect. The "term significance" table shows effect of individual factors (or interactions).

Method used to set up biofilters on a surface in the field

How biofilters are set up.

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