Richard Lab:protocols: Difference between revisions
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=Methods= | =Methods= | ||
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==Biomass | ==Biomass Protocols== | ||
::'''[[Silage protocols|Silage Protocols]]''' | ::'''[[Silage protocols|Silage Protocols]]''' | ||
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::'''[[Richard Lab:Enzymatic Hydrolysis of Biomass|Enzymatic Hydrolysis of Biomass]]''' | ::'''[[Richard Lab:Enzymatic Hydrolysis of Biomass|Enzymatic Hydrolysis of Biomass]]''' | ||
::'''[[Quantification of glucose|Quantification of Glucose]]''' | ::'''[[Quantification of glucose|Quantification of Glucose]]''' | ||
::'''[[Richard Lab:SSCF|Simultaneous Saccharification and Co-Fermentation]]''' | |||
==Enzymatic Screens and Assays== | ==Enzymatic Screens and Assays== | ||
Revision as of 12:31, 12 September 2011
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Equipment
Dionex IC
Dionex ASE
Anaerobic Chamber
YSI Analyzer
Methods
Biomass Protocols
- Silage Protocols
- Biomass Compositional Analysis
- NDF (Neutral Detergent Fiber) Determination
- ADF (Acid Detergent Fiber) Determination
- ADL (Acid Detergent Lignin) Determination
- Dilute-Acid Pretreatment of Biomass
- Enzymatic Hydrolysis of Biomass
- Quantification of Glucose
- Simultaneous Saccharification and Co-Fermentation
Enzymatic Screens and Assays
Molecular/Synthetic Biology
- Amplified Insert Assembly
- Bio-Brick Formats
- Richard Lab PCR Protocols
- Site Directed Mutagenesis
- Plasmid Extraction
- DNA Quantification
- DNA Concentration
- DNA Synthesis
- Restriction Digestion
- Partial Restriction Digest
- Agarose Gel Electrophoresis
- Polyacrylamide Gel Electrophoresis
- Gel Extraction of DNA
- Ligation
- Preparation of Shuttle-vectors for transformation
- Transforming Lactococcus lactis
- Making Electro-competent E. coli
- Electro-transformation of E. coli
- Electro-transformation of L. Plantarum
- Chromosomal Integration
Manure Storage and Emissions Analysis
- Measuring and Analyzing Gas-Flux from a Surface
- Setting Up Biofilters in the Field.
- Formatting Data from Data Logger
- The following is a set of instructions on how to use macros to transform a data set from configuration 1 to configuration 2.
- BOD, moisture and VS content
- Organic matter samples are analyzed according to the following instructions for BOD (Biological Oxygen Demand) and moisture and Volatile Solids (organic matter) content analysis.
Compost Analysis
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