Difference between revisions of "Richard Lab:protocols"

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(Compost Analysis)
(Enzymatic Screens and Assays)
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==Enzymatic Screens and Assays==
==Enzymatic Screens and Assays==
::'''Ferulic-Acid Esterase Screen'''
::'''Ferulic-Acid Esterase Screen'''
::'''Ferulic Acid Esterase Assay'''
::'''[[Feruloyl Esterase Protocols|Ferulic Acid Esterase Protocols]]'''
::'''Laccase Plate Screen'''
::'''Laccase Plate Screen'''
::'''Laccase Assay'''
::'''Laccase Assay'''

Revision as of 16:27, 26 January 2011

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Dionex IC

Eluent Preparation
Standard Preparation

Dionex ASE


Anaerobic Chamber


Molecular/Synthetic Biology

Bio-Brick Formats
Richard Lab PCR Protocols
Site Directed Mutagenesis
New Standard Assembly
Plasmid Extraction
DNA Quantification
DNA Concentration
DNA Synthesis
Restriction Digestion
Partial Restriction Digest
Agarose Gel Electrophoresis
Polyacrylamide Gel Electrophoresis
Gel Extraction of DNA
Preparation of Shuttle-vectors for transformation
Making Electro-competent E. coli
Electro-transformation of E. coli
Making Electro-competent L. plantarum
Electro-transformation of L. Plantarum
Chromosomal Integration

Enzymatic Screens and Assays

Ferulic-Acid Esterase Screen
Ferulic Acid Esterase Protocols
Laccase Plate Screen
Laccase Assay
Xylanase Plate Screen
Xylanase Assay

Biomass Analysis

Mini-Silo Sampling
NDF (Neutral Detergent Fiber)
ADF (Acid Detergent Fiber)
ADL (Acid Detergent Lignin)
Acetyl Bromide Lignin Analysis
TMAH Lignin Analysis
Enzymatic Hydrolysis of Biomass
Quantification of Glucose

Manure Storage and Emissions Analysis

Measuring and Analyzing Gas-Flux from a Surface
Setting Up Biofilters in the Field.
Formatting Data from Data Logger
BOD, moisture and VS content

Compost Analysis

Manure and Compost Sample Preparation
Chemical Oxygen Demand

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