Difference between revisions of "Richard Lab:ADL"

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(Notes)
(Materials)
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==Materials==
 
==Materials==
List reagents, supplies and equipment necessary to perform the protocol here.  For those materials which have their own OWW pages, link to that page.  Alternatively, links to the suppliers' page on that material are also appropriate.
 
  
*supply 1 (i.e. tubes of a certain size? spreaders?)
+
*2000 mL flask
*reagent 1
+
*acetone (in flammables cabinet below fume hood in room 113B)
*X μL reagent 2
+
*5 glass jars
**component A (reagent 2 is made up of multiple components)
+
*microwave
**component B
+
*pH paper
*equipment 1
+
*concentrated sulfuric acid
*equipment 2
+
*top-loading balance (1 decimal place)
 +
*ANKOM Daisy incubator and jars
 +
*analytical balance
 +
*drying oven at 105oC
 +
*dessicator
 +
*zip-loc bags with dessicant pouches (recently recharged at 105oC)
 +
*hazardous waste containers
  
 
==Procedure==
 
==Procedure==

Revision as of 12:31, 4 June 2009

Overview

Replace this sentence with a brief description of the protocol and its goal.

Materials

  • 2000 mL flask
  • acetone (in flammables cabinet below fume hood in room 113B)
  • 5 glass jars
  • microwave
  • pH paper
  • concentrated sulfuric acid
  • top-loading balance (1 decimal place)
  • ANKOM Daisy incubator and jars
  • analytical balance
  • drying oven at 105oC
  • dessicator
  • zip-loc bags with dessicant pouches (recently recharged at 105oC)
  • hazardous waste containers

Procedure

  1. Step 1
  2. Step 2
    • Step 2 has some additional information that goes with it. i.e. Keep at 4°C.
  3. Step 3
    1. Step 3 has multiple sub-steps within it.
    2. Enumerate each of those.

Notes

  • ALWAYS add acid to water and work in fume hood when making 72% sulfuric acid

References

Relevant papers and books

  1. Goldbeter A and Koshland DE Jr. An amplified sensitivity arising from covalent modification in biological systems. Proc Natl Acad Sci U S A. 1981 Nov;78(11):6840-4. PubMed ID:6947258 | HubMed [Goldbeter-PNAS-1981]
  2. JACOB F and MONOD J. Genetic regulatory mechanisms in the synthesis of proteins. J Mol Biol. 1961 Jun;3:318-56. PubMed ID:13718526 | HubMed [Jacob-JMB-1961]
  3. ISBN:0879697164 [Ptashne-Genetic-Switch]
All Medline abstracts: PubMed | HubMed

Contact

  • Who has experience with this protocol?

or instead, discuss this protocol.