Richard Lab:ADL: Difference between revisions
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==Materials== | ==Materials== | ||
* | *2000 mL flask | ||
* | *acetone (in flammables cabinet below fume hood in room 113B) | ||
* | *5 glass jars | ||
** | *microwave | ||
** | *pH paper | ||
* | *concentrated sulfuric acid | ||
* | *top-loading balance (1 decimal place) | ||
*ANKOM Daisy incubator and jars | |||
*analytical balance | |||
*drying oven at 105oC | |||
*dessicator | |||
*zip-loc bags with dessicant pouches (recently recharged at 105oC) | |||
*hazardous waste containers | |||
==Procedure== | ==Procedure== | ||
Revision as of 13:31, 4 June 2009
Overview
Replace this sentence with a brief description of the protocol and its goal.
Materials
- 2000 mL flask
- acetone (in flammables cabinet below fume hood in room 113B)
- 5 glass jars
- microwave
- pH paper
- concentrated sulfuric acid
- top-loading balance (1 decimal place)
- ANKOM Daisy incubator and jars
- analytical balance
- drying oven at 105oC
- dessicator
- zip-loc bags with dessicant pouches (recently recharged at 105oC)
- hazardous waste containers
Procedure
- Step 1
- Step 2
- Step 2 has some additional information that goes with it. i.e. Keep at 4°C.
- Step 3
- Step 3 has multiple sub-steps within it.
- Enumerate each of those.
Notes
- ALWAYS add acid to water and work in fume hood when making 72% sulfuric acid
References
Relevant papers and books
- Goldbeter A and Koshland DE Jr. An amplified sensitivity arising from covalent modification in biological systems. Proc Natl Acad Sci U S A. 1981 Nov;78(11):6840-4. DOI:10.1073/pnas.78.11.6840 |
- JACOB F and MONOD J. Genetic regulatory mechanisms in the synthesis of proteins. J Mol Biol. 1961 Jun;3:318-56. DOI:10.1016/s0022-2836(61)80072-7 |
- ISBN:0879697164
Contact
- Who has experience with this protocol?
or instead, discuss this protocol.
