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| ==Abstract==
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| This protocol can be used to generate genomic DNA fragments of different sizes using a common cutter. This is useful for many screening/probing processes.
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| ==Materials==
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| * 10 microcentrifuge tubes
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| ===Reagents===
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| * Restriction Endonuclease
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| * 10X Restriction Endonuclease buffer
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| * BSA (10 mg/mL)
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| * Genomic DNA (Concentration > 0.1 μg/μL)
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| ==Procedure==
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| # Label the microcentrifuge tubes 1 - 10
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| # Mix together the following and invert to mix:
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| ## 180μL Genomic DNA
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| ## 20μL 10X Buffer
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| ## 2μL BSA
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| # Dispense 40μL of this mix into tube 1 and 20μL into tubes 2 - 10 **[[Image:Critical step.png]]'''From this point keep everything on ice!!!'''
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| # Add 1μL of restriction-endonuclease to tube 1 and invert to mix.
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| # Transfer 20μL from tube 1 to tube 2 and invert to mix.
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| # Repeat the last step transfering 20μL from the preceding tube to the succeeding tube mixing with every transfer.
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| # Finally remove 20μL from tube 10
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| # Incubate all 10 tubes for 1 hour at 37°C.
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| # Finish the digest by submerging all 10 tubes in an 70°C water bath for 20 minutes.
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| #Run the samples on a gel and choose the size which best fits your application.
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| ==Acknowledgments==
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| Acnkowledge any help you had in development, testing, writing this protocol.
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| ==References==
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| See [[OpenWetWare:Biblio]] for information on how to reference within a wiki.
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| ==Specific Protocols==
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| Add links to all the OWW protocols that have been used in making the consensus.
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| ==Discussion==
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| You can [[Talk:{{PAGENAME}}|discuss this protocol]].
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| [[Category:Protocol]]
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| [[Category:In vitro]]
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| [[Category:DNA]]
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