Difference between revisions of "Reiter:IPTG"

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(New page: {{Template:Reiter}} == 1 M IPTG stock preparation== * 10 g IPTG * 41.960 mL sterile H<sub>2</sub>O # Vortex or shake until completely dissolved. # 0.22 μm filter into new 50 mL tube. # ...)
 
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Latest revision as of 14:47, 5 March 2012

Reiter.PNG

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1 M IPTG stock preparation

  • 10 g IPTG
  • 41.960 mL sterile H2O
  1. Vortex or shake until completely dissolved.
  2. 0.22 μm filter into new 50 mL tube.
  3. Dispense 1 mL aliquots and freeze at -20 °C